Vitellogenin induction has been widely used as a biomarker of endocrine disruption in wildlife, but few studies have investigated its use in wild reptiles living in contaminated habitats. This study examined vitellogenin induction in Morelet's crocodiles (Crocodylus moreletii) from wetlands in northern Belize contaminated with organochlorine (OC) pesticides. Vitellogenin was measured in 381 cro...

Vitellogenin is a yolk precursor protein in most oviparous females. In the advanced eusocial honeybee, Apis mellifera (Hymenoptera: Apidae), vitellogenin has recently attracted much interest as this protein, in addition to a classical function in oocyte development in the reproductive queen caste, has evolved functions in the facultatively sterile female worker caste not documented in other spe...

2 Yolk protein chemistry 90 2.1 Table of yolk protein properties 90 2.2 Vitellin, the major yolk protein 90 2.2.1 Vitellogenin amino acid composition 91 2.2.2 Vitellogenin peptide compositions 94 2.2.3 Vitellogenin oligosaccharide 95 2.2.4 Microheterogeneity of vitellin oligosaccharide 96 2.2.5 Vitellogenin lipids 97 2.2.6 Vitellin phosphate 98 2.3 Lipophorin 98 2.3.1 Lipophorin properties 98 2...

Direct induction of vitellogenin production in cultured male amphibian hepatocytes by estradiol-17 beta has been accomplished. Liver cells were isolated from adult male bullfrogs by collagenase perfusion and maintained as monolayers in serum-free medium containing insulin and estradiol. Vitellogenin production was measured by direct immunoprecipitation from radioactively labeled secreted protei...

Vitellogenin, the precursor of egg yolk phosphoproteins, is synthesized in the liver of the laying hen but is not normally synthesized by roosters. Administration of 17/?estradiol to roosters induces the synthesis of large amounts of this protein and its messenger RNA. We have purified vitellogenin messenger RNA from the livers of estrogentreated roosters and have characterized it as follows: (...

A sensitive optical waveguide lightmode spectroscopy-based immunosensor was developed to detect vitellogenin in seawater flatfish (Paralichthys olivaceus). For this purpose, anion-exchange column chromatography with DE-52 resin was used to purify flatfish vitellogenin from flatfish serum containing vitellogenin that had been induced using an intraperitoneal 17beta-estradiol injection. The anti-...

The gene for vitellogenin, an egg yolk protein precursor, is usually silent in male fish but can be induced by estrogen exposure. For this reason, vitellogenin production in male fish has become a widely used indicator of exposure to exogenous estrogens or estrogen mimics in the aquatic environment. The utility of this indicator to predict impacts on fish reproductive success is unclear because...

Protection against inflammation and oxidative stress is key in slowing down aging processes. The honey bee (Apis mellifera) shows flexible aging patterns linked to the social role of individual bees. One molecular factor associated with honey bee aging regulation is vitellogenin, a lipoglycophosphoprotein with anti-inflammatory and antioxidant properties. Recently, we identified three genes in ...

Administration of estrogen to roosters induces the synthesis of egg yolk phosphoproteins in the liver. We have demonstrated that these proteins are synthesized in the form of a large precursor, vitellogenin, and that vitellogenin is the only phosphoprotein found in the plasma of the estrogen-treated rooster. Vitellogenin is cleaved to form the egg yolk phosphoproteins, lipovitellin, and phosvit...

In the cockroach Blattella germanica, the synthesis of vitellogenin is juvenile hormone III (JH III)-dependent. We have studied the effect of JH III upon vitellogenin gene expression in periovaric fat bodies incubated in vitro. Periovaric fat bodies were obtained from cardioallatectomized females. The response to JH III was measured in terms of vitellogenin and vitellogenin mRNA after 7 h of in...