Adeno-associated virus (AAV) is a single-stranded parvovirus retaining the unique capacity for site-specific integration into a transcriptionally silent region of the human genome, a characteristic requiring the functional properties of the Rep 78/68 polypeptide in conjunction with AAV terminal repeat integrating elements. Previous strategies designed to assemble these genetic elements into ade...

Rapid and sensitive methods for accurate strain delineation are essential for monitoring and preventing transmission of methicillin-resistant Staphylococcus aureus (MRSA). Pulsed-field gel electrophoresis (PFGE) has been the standard technique for strain typing most bacterial species including MRSA. The goal of this study was to compare the performance of the DiversiLab microbial typing system ...

Replication initiation of nanoviruses, plant viruses with a multipartite circular single-stranded DNA genome, is triggered by the master Rep (M-Rep) protein. To enable the study of interactions between M-Rep and viral or host factors involved in replication, we designed oligohistidine-tagged variants of the nanovirus Faba bean necrotic yellows virus (FBNYV) M-Rep protein that allow affinity pur...

Using a polyclonal antiserum a cDNA encoding a Rab escort protein (REP) homolog in Drosophila has been identified and sequenced. The gene encodes a 511 residue protein with a predicted molecular mass of 56855 Da. Antibody labeling demonstrates that Drosophila REP protein is present in the early embryo and that it is being apportioned uniformly throughout the embryo in a process likely to be lin...

The Escherichia coli Rep protein is a DNA helicase that is involved in DNA replication. We have examined the effects of DNA binding on the assembly state of the Rep protein using small-zone gel permeation chromatography and chemical crosslinking of the protein. Complexes of Rep protein were formed with short single-stranded and duplex hairpin oligodeoxynucleotides with lengths such that only a ...

32 Recently the DiversiLab (DL) system (bioMérieux) was developed as an automated platform that uses 33 rep-PCR technology for standardized, reproducible DNA fingerprinting of bacteria. The purpose of this 34 study was to evaluate the usefulness of DL rep-PCR for typing of Pseudomonas aeruginosa isolates. 35 The performance of DL rep-PCR was compared with that of pulsed-field gel electrophoresi...

background: fusobacterium necrophorum as a non-spore-forming gram-negative anaerobic bacillus is an important human and animal pathogen. it may cause severe systemic infections (lemierre's syndrome) and some other infections. the aim of this study was to subtype fusobacterium necrophorum by using pcr methods. materials and methods: twenty five strains of fusobacterium necrophorum subspecies fun...

forty monoconidial isolates of magnaporthe grisea were examined, for an identification of vegetative compatibility group and a characterization of genetic diversity, using rep-pcr genomic fingerprinting. the isolates were collected from weeds digitaria sanguinalis (crabgrass), setaria italica (foxtail millet), echinochloa crus-galli (barnyard millet), and some other unknown ones during 2003 - 2...