molecular characterization of 19 jatropha accessions that included 15 accessions of j.curcas and 4 different species was carried out using 3 different markers systems. highest polymorphism (96.67%) was recorded by rapd followed by damd (91.02%) and issr (90%). polymorphism information content (pic) was higher for damd (0.873) and almost equal for rapd (0.863) and issr (0.862) markers, whereas r...

The genetic diversity in Zanthoxylum species viz.  Zanthoxylum nitidum, Zanthoxylum oxyphyllum and Zanthoxylum rhesta collected from the Upper Brahmaputra Valley Zone of Assam (NE India) was amplified using 13 random amplified polymorphic DNA (RAPD) markers and 9 inter-simple sequence repeat (ISSR) markers. RAPD markers were able to detect 81.82% polymorphism whereas ISSR detected 98.02% polymo...

Genetic and epigenetic changes (DNA methylation) in the mother plants of somatic hybrids (1st cycle) and their regenerants (30th cycles sub-cultured) were analyzed using methylation-sensitive ISSR and RAPD markers. ISSR and RAPD profiles and their cluster analyses based on the Jaccard’s similarity coefficient revealed 100% genetic similarity among the mother plant and their regenerants. While f...

We described the use of the random amplified polymorphic DNA (RAPD) technique on Plasmodium falciparum DNA to detect genetic markers for chloroquine-resistant strains. Fourteen RAPD primers were tested, three of which generated banding patterns correlated with chloroquine resistance. To measure this correlation, the RAPD profiles were analyzed using the Nei and Li similarity coefficient. Detect...

Molecular characterization of 19 Jatropha accessions that included 15 accessions of J.curcas and 4 different species was carried out using 3 different markers systems. Highest polymorphism (96.67%) was recorded by RAPD followed by DAMD (91.02%) and ISSR (90%). Polymorphism Information Content (PIC) was higher for DAMD (0.873) and almost equal for RAPD (0.863) and ISSR (0.862) markers, whereas R...

Morphologically, nine different slow-growing protoclones were screened from regenerated protoplast of heterokaryotic Agaricus bisporus. The present study is the first report of fingerprinting on differentiating homo- and heterokaryotic protoclones using random amplified polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR) markers. Among 80 primers tested, the seven ISSR and seven RAPD...

Due to advances in molecular biology techniques, large numbers of highly informative DNA markers have been developed for the identification of genetic polymorphism. In the last decade, the random amplified polymorphic DNA (RAPD) technique based on the polymerase chain reaction (PCR) has been one of the most commonly used molecular techniques to develop DNA markers. RAPD markers are amplificatio...

We produced 102 randomly amplified polymorphic DNA (RAPD) markers mapped on all 12 chromosomes of rice using DNAs of cultivars Nipponbare (japonica) and Kasalath (indica) and of F2 population generated by a single cross of these parents. Sixty random primers 10 nucleotides long were used both singly and in random pairs and about 1,400 primer-pairs were tested. Using both agarose gel and polyacr...

Using only one type of marker to quantify genetic diversity generates results that have been questioned in terms of reliability, when compared to the combined use of different markers. To compare the efficiency of the use of single versus multiple markers, we quantified genetic diversity among 10 S(7) inbred popcorn lines using both RAPD and SSR markers, and we evaluated how well these two type...