Eight rumen-fistulated steers were randomly assigned to mediumand high-concentrate diets supplemented with O, .75, 2.5 and 5% of either sodium bicarbonate or an artificial saliva salts mixture. Each animal was fed for 21 d at 75 g dry matter/kg body weight 'Ts. Rates of soybean meal (SBM) degradation were predicted by adjusting digestion rates in nylon bags with respective passage rates of chro...

Salt concentration governs nucleic acid hybridization according to the Schildkraut-Lifson (1965) equation. High concentrations of sodium dodecyl sulfate (SDS) are used in some common protocols (Church and Gilbert, 1984; Virca et al., 1990) but the effects of SDS on hybridization stringency have not been reported. We investigated hybridization parameters in solutions containing SDS. With targets...

Four sheep, each fitted with cannulas in the rumen and proximal duodenum, were given two diets (1390 g dry matter (DM)/d) consisting of lucerne (Medicago sativa) pellets (24.2 g nitrogen/kg DM) plus pelleted reed canary grass (Phalaris arundinacea; 14.1 g N/kg DM) or chopped hay (11.8 g N/kg DM) at intervals of 2 h. Flow of duodenal digesta measured by reference to the markers 51Cr-EDTA and 103...

Intensive harvesting of forest residues for energy production may lead to the depletion of organic matter and mineral nutrients in the forest floor. In order to restore nutrient content wood ash has been suggested as a fertiliser. Ectomycorrhizal (EM) fungi are involved in the nutrient uptake of forest trees and this study investigates the influence of intensive harvesting and wood ash fertilis...

This study was conducted to evaluate the effect of electron beam irradiation at doses of 250 and 500 kGy on the chemical composition and ruminal dry matter (DM) and neutral detergent fiber (NDF) degradability of wheat straw. Nylon bags of untreated or irradiated wheat straw were suspended in the rumen of three rams for up to 72 h, and resulting data were fitted to non-linear degradation model t...

Three techniques for estimating rumen protein degradability were used together with in vivo apparent nitrogen digestibility estimates from sheep fed dried chopped Pangola grass or Siratro. The techniques used were 1) nitrogen disappearance from nylon bags suspended in the rumen, 2) S labelling of rumen microbial protein with plant nitrogen degradability calculated as the difference between micr...

The effect of whole lupin seeds (Lupinus albus cv Lublanc) at 120, 150 and 195 degrees C on in situ nitrogen degradability (Dg.N) was measured by the nylon bag technique using fistulated non-lactating Holstein cows. The N degradation was evaluated in nylon bags suspended in the rumen; heating the seeds at 120, 150 and 195 degrees C decreased the Dg.N value: 83.9, 72.9 and 53.0 respectively vs 9...

this study was conducted to evaluate the effect of electron beam irradiation at doses of 250 and 500 kgy on the chemical composition and ruminal dry matter (dm) and neutral detergent fiber (ndf) degradability of wheat straw. nylon bags of untreated or irradiated wheat straw were suspended in the rumen of three rams for up to 72 h, and resulting data were fitted to non-linear degradation model t...