The simple titration method for the determination of organic acids in urine, described by Van Slyke and Palmer (I), has in many circumstances proved satisfactory. A more extended employment of the method to determine organic acids in pathological urines has brought to light limitations and sources of error which need consideration. The organic acids present in urine, both free and as salts, are...

The simple titration method for the determination of organic acids in urine, described by Van Slyke and Palmer (I), has in many circumstances proved satisfactory. A more extended employment of the method to determine organic acids in pathological urines has brought to light limitations and sources of error which need consideration. The organic acids present in urine, both free and as salts, are...

Acidosis is an important cause of mortality in severe falciparum malaria. Lactic acid is a major contributor to metabolic acidosis, but accounts for only one-quarter of the strong anion gap. Other unidentified organic acids have an independent strong prognostic significance for a fatal outcome. In this study, a simultaneous bio-analytical method for qualitative and quantitative assessment in pl...

Background: Urinary organic acids are water-soluble intermediates and end products of the metabolism of amino acids, carbohydrates, lipids, and a number of other metabolic processes. In the hereditary diseases known as organic acidurias, an enzyme or co-factor defect in a metabolic pathway leads to the accumulation and increased excretion of one or more of these acidic metabolites. Gas chromato...

background: urinary organic acids are water-soluble intermediates and end products of the metabolism of amino acids, carbohydrates, lipids, and a number of other metabolic processes. in the hereditary diseases known as organic acidurias, an enzyme or co-factor defect in a metabolic pathway leads to the accumulation and increased excretion of one or more of these acidic metabolites. gas chromato...

organic acidemias, also known as organic acidurias, are a group of disorders characterized by increased excretion of organic acids in urine. they result primarily from deficiencies of specific enzymes in the breakdown pathways of amino acids or from enzyme deficiencies in beta oxidation of fatty acids or carbohydrate metabolism. organic acids also are found in the urine of some patients with mi...

We investigated the use of a diode array detector in conjunction with isocratic cation-exchange liquid chromatography for detection of organic acids in urine. The spectra and retention times of abnormal peaks found on chromatography of urine from patients with methylmalonic aciduria, maple syrup urine disease, and lactic aciduria were recorded and compared with those obtained for group of pure ...

The determination ofthe so-called 'ethereal sulphate content' of urine is a simple analytical procedure, and there are many reports in the literature which show that the administration of phenols to experimental animals is followed by an increased excretion ofethereal sulphates in the urine. On the other hand, because of the difficulties which have usually been encountered, relatively few ether...

A gas-chromatographic method for urinary organic acid analysis is described, designed to be used routinely for the diagnosis of organic aciduria. It involves extraction of urine with ethyl acetate, dehydration of extract residues, and trimethylsilylation. Organic acids are identified by using an extensive list of retention indices published in the accompanying paper (this issue). Quantitative v...

BACKGROUND Maple syrup urine disease (MSUD) is the most common inborn error of metabolism in the country. The cause of the neuropathology is still not well established although accumulation of branched chain amino acids (BCAA) and alteration in large neutral amino acids (LNAA) as well as energy deprivation are suggested. It is therefore the aim of this study to determine the plasma amino acid a...