Derivatives of UMP (uridine monophosphate) with a fluorogenic substituent in position 5 represent a small but unique class of fluorophores, which has found important applications in chemical biology and biomolecular chemistry. In this study, we have synthesised a series of derivatives of the uracil nucleotides UMP, UDP and UTP with different aromatic and heteroaromatic substituents in position ...

The aim of the present study was to investigate in vivo toxic effects and to find acute toxic doses (LD50) of novel N-sulfonyl derivatives of pyrimidine nucleobases uracil and cytosine. Six N-1-sulfonyluracil derivatives (1, 2, 6, 7, 8, and 9) and two N-1-sulfonylcytosine (4, 12) and N-1,NH-4-disulfonylcytosine (13) were evaluated in this study. All experiments were performed on 10-14week-old m...

Molecular docking method was applied to research steric complementarity of 27 uracil derivatives modified into positions N, N, C, C by cyclic and acyclic substituents with the active site of thymidylate synthetase of mice Mus musculus. AutoDock 4.2 scoring function evaluated the thermodynamic characteristics of all compounds’ binding with the active site of the given enzyme. 17 uracil derivativ...

The synthesis of the pyrimidinyl amino acids willardiine and isowillardiine was studied in vivo and in vitro. Uracil derivatives stimulate the biosynthesis of both compounds; the free base is the most effective. Significant incorporation of [2-(14)C]uracil and [6-(14)C]orotate into willardiine and isowillardiine was found. Incorporation of [6-(14)C]orotate was substantially decreased in the pre...

Uracil DNA N-glycosylase is a repair enzyme that releases uracil from DNA. A major function of this enzyme is presumably to protect the genome from pre-mutagenic uracil resulting from deamination of cytosine in DNA. Here, we report that human uracil DNA N-glycosylase also recognizes three uracil derivatives that are generated as major products of cytosine in DNA by hydroxyl radical attack or ot...

A series of potentially biologically active fluorinated uracil derivatives has been prepared in three steps from oxazolines and fluorinated nitriles with good chemical yields.

The wobble hypothesis does not discriminate between uracil and thymine. Methylation could favor further stabilization of uracil in the keto form. Thymine is present in keto form only and can pair up but with adenine. Uracil can easily construct the enol form; that is why it forms the U-G pair. In previous E-print I proposed an alternative to the wobble hypothesis [1]. According to my reviewers ...

The excited state properties of uracil, thymine and four analogous uracil compounds have been studied in acetonitrile by steady-state and time-resolved spectroscopy. The excited state lifetimes were measured using femtosecond UV fluorescence upconversion. The excited state lifetimes of uracil and its 1and 3-methyl substituted derivatives are well described by one ultrafast (6100 fs) component. ...