Coagulation factor V (FV), present in plasma and platelets, is indispensable to thrombin formation, yet patients with undetectable plasma FV seldom experience major bleeding. We used thrombin generation assays to explore the role of platelet FV in 4 patients with severe congenital FV deficiency (3 with plasma FV clotting activity [FV:C] < 1%). When triggered with tissue factor (TF) concentratio...

Coagulation factor V (FV) is a central regulator of the coagulation cascade. Circulating FV is found in plasma and within platelet alpha granules. The specific functions of these distinct FV pools are uncertain. We now report the generation of transgenic mice with FV gene expression restricted to either the liver or megakaryocyte/platelet lineage using bacterial artificial chromosome (BAC) cons...

A missense mutation, FV-Ile359Thr (FV Liverpool), associated with thrombosis has recently been described. This mutation creates an additional potential Nlinked glycosylation site (Asn-X-Ser/Thr) in factor V (FV) at Asn357 that could interfere with secretion and/or protein interactions. To investigate the molecular pathology of FV-Ile359Thr, the mutation was created by site-directed mutagenesis ...

BACKGROUND Multi-component interventions which combine educational and environmental strategies appear to be most effective in increasing fruit and vegetable (FV) intake in adolescents. However, multi-component interventions are complex to implement and often poorly implemented. Identification of barriers and facilitators for implementation is warranted to improve future interventions.This stud...

OBJECTIVE To investigate the frequency of unilateral or bilateral foramen of Vesalius (FV), number of FV present on one side, and presence of a septum on FV. METHODS We bilaterally examined, 347 sphenoid bones of collections of the Department of Anatomy, Istanbul Faculty of Medicine, Istanbul University, Istanbul and Dokuz Eylul Faculty of Medicine, Izmir, Turkey in 2007. RESULTS Of the 347...

A missense mutation, FV-Ile359Thr (FV Liverpool), associated with thrombosis has recently been described. This mutation creates an additional potential N-linked glycosylation site (Asn-X-Ser/Thr) in factor V (FV) at Asn357 that could interfere with secretion and/or protein interactions. To investigate the molecular pathology of FV-Ile359Thr, the mutation was created by site-directed mutagenesis...

Factor V (FV), a central regulatory protein in hemostasis, is distributed into distinct plasma and platelet compartments. Although platelet FV is highly concentrated within the platelet alpha-granule, previous analysis of human bone marrow and liver transplant recipients has demonstrated that platelet FV in these individuals originates entirely from the uptake of plasma FV. In order to examine ...

Background—Factor V (fV) predisposes to thrombosis by enhancing thrombin formation. This study tested the hypothesis that fV inhibits fibrinolysis in vivo. Methods and Results—Radiolabeled clots were injected into the jugular veins of wild-type mice and mice heterozygous (fV ) or homozygous (fV) for fV. Mean percent clot lysis 5 hours later was significantly reduced in fV mice (14.3 3.6%, n 13)...

Recall that some of the substitution rules mentioned the function FV : {λ-terms} → Var: FV(x) = {x} FV(e1 e2) = FV(e1) ∪ FV(e2) FV(λx. e) = FV(e)− {x}. Why does this definition uniquely determine the function FV? There are two issues here: • Existence: whether FV is defined on all λ-terms; • Uniqueness: whether the definition is unique. Of relevance here is the fact that there are three clauses...

In antigen-antibody interactions, the high avidity of antibodies for their specific antigens can be achieved both by exploiting the high affinity of each binding site and by multivalence of antibodies. In this study, we developed artificial antibodies with multiple valency. The Fv fragments of an antibody fused with one and with two domains of the Fc-binding protein A from Staphylococcus aureus...