Reductive methylation has been used to radioactively label crude-extract proteins with 3H or 14C. The procedure achieved good isotope incorporation and resolution of proteins on two-dimensional polyacrylamide gels. It allows high-precision comparison of tissue samples by double-labelling and should facilitate the study of tissue proteins by two-dimensional gel electrophoresis.

in this paper, the two-dimensional triangular orthogonal functions (2d-tfs) are applied for solving a class of nonlinear two-dimensional volterra integral equations. 2d-tfs method transforms these integral equations into a system of linear algebraic equations. the high accuracy of this method is verified through a numerical example and comparison of the results with the other numerical methods.

INTRODUCTION SDS-polyacrylamide gel electrophoresis (SDS-PAGE) represents the second-dimension separation of two-dimensional (2D)-PAGE. Large-scale proteome analysis usually requires simultaneous electrophoresis of batches of second-dimension SDS-PAGE gels to maximize the reproducibility of 2D electrophoresis protein profiles. This requirement is most easily met using multiple, vertical second-...

Two-dimensional gel electrophoresis (2-DE) methods such as two-dimensional polyacrylamide gel electrophoresis (2D-PAGE; O’Farrell (1975)) and two-dimensional difference gel electrophoresis (2D-DIGE; Ünlü et al. (1997)) are popular techniques for protein separation because they allow researchers to characterize quantitative protein changes on a large scale. Thus, 2-DE is frequently used as an in...

Having knowledge of stability of an underground space depends on stresses and strains around it. Creating underground tunnels leads to significant changes in the rock mass stress. Therefore, to achieve the necessary stability, stresses and deformations around the tunnel must be examined carefully. Usually, stress-strain behavior analysis is conducted in two-dimensional mode. This paper was cond...

the indian riverine buffaloes are more susceptible to variations in the environment. these variations affect seminal plasma proteins, which in turn affect fertility. this study was conducted to evaluate variations in the indian bubalus bubalis seminal plasma protein profile using two-dimensional polyacrylamide gel electrophoresis (2d-page) during winter and summer seasons, and its relation with...

‎In this paper‎, ‎we introduce the two-dimensional Legendre wavelets (2D-LWs)‎, ‎and develop them for solving a class of two-dimensional integro-differential equations (2D-IDEs) of fractional order‎. ‎We also investigate convergence of the method‎. ‎Finally‎, ‎we give some illustrative examples to demonstrate the validity and efficiency of the method.