Tulip (Tulipa gesneriana L.) is a bulbous plant species that requires a period of low temperature for proper growth and flowering. The mechanism of sensing the low temperature period is unknown. The study presented in this paper shows that the essential developmental change in tulip bulbs during cold treatment is an increase in sensitivity to the phytohormone auxin. This is demonstrated using a...

Tulip is one of the most important bulbous genera in world’s floriculture. It known that cold exposure bulbs before planting required to break bulb dormancy and promote plant’s flowering. Preparation procedures performed by breeders differ duration thermal level, choice procedure depends on genotype’s sensitivity temperature; however, little about metabolic responses underlying different behavi...

In recent years, tulip flowers have been grown extensively in parks and green spaces of Urmia city (NW Iran). During the surveys in a period from 2014–16, characteristic symptoms of the blue mold rot were observed on both planted and stored tulip bulbs, as the bulbs were covered with a white to bluish-green mycelia and spores. In order to determine the fungi associated with tulip bulbs (Tulipa ...

Five cDNA clones encoding 1-aminocyclopropane-1-carboxylic acid (ACC) oxidase (ACO) were isolated from tulip (Tulipa gesneriana L.) by differential screening of the petal cDNA library and were designated as TgACO1, TgACO2, TgACO3, TgACO4 and TgACO5. The deduced amino acid sequences exhibited similarity to ACO proteins from other plant species. Among these proteins, TgACO1-4 have high similarity...

Sucrose (Suc):Suc 1-fructosyltransferase (1-SST) is the key enzyme in plant fructan biosynthesis, since it catalyzes de novo fructan synthesis from Suc. We have cloned 1-SST from onion (Allium cepa) by screening a cDNA library using acid invertase from tulip (Tulipa gesneriana) as a probe. Expression assays in tobacco (Nicotiana plumbaginifolia) protoplasts showed the formation of 1-kestose fro...

We developed capillary affinity electrophoresis (CAE) to analyze the molecular interaction between carbohydrate chains and proteins in solution state. A mixture of oligosaccharides derived from a glycoprotein was labeled with 8-aminopyrene-1,3,6-trisulfonate (APTS), and used as glycan library without isolation. Interaction of a carbohydrate-binding protein with each oligosaccharide in the mixtu...