The polypeptide chain elongation factor EF-Tu of Escherichia coli is encoded by two genes, tufA and tufB, located in two different operons. Experiments in which either tufA or tufB was inactivated demonstrated that expression of the tRNA-tufB operon is dependent on a functioning tufA and thus on EF-Tu (1, to be published). In order to study a possible role of EF-Tu as trans-activator of the tRN...

Microalgae identification is extremely difficult. The efficiency of DNA barcoding in microalgae identification involves ideal gene markers and approaches employed, which however, is still under the way. Although Scenedesmus has obtained much research in producing lipids its identification is difficult. Here we present a comprehensive coalescent, distance and character-based DNA barcoding for 11...

Probes derived from the tufA gene of Escherichia coli have been utilized to detect homologous sequences on Spirulina platensis DNA. A 6-kilobase-pair fragment of S. platensis DNA appears to contain two sequences homologous to the E. coli gene. Thus, as reported for gram-negative bacteria, the cyanobacterium presumably contains two tuf genes.

The Ikka Fjord (SW Greenland) harbors a unique microbial habitat in the form of several hundred submarine tufa columns composed of ikaite, a special hexahydrate form of calcium carbonate that precipitates when alkaline phosphate- and carbonate-enriched spring water seeping out of the sea floor meets cold seawater. While several unique heterotrophic microbes have been isolated from the tufa colu...

The data presented here are related to the research article "Multi-marker metabarcoding of coral skeletons reveals a rich microbiome and diverse evolutionary origins of endolithic algae" (Marcelino and Verbruggen, 2016) [1]. Here we provide reference datasets of the elongation factor Tu (tufA) and the Universal Plastid Amplicon (UPA) markers in a format that is ready-to-use in the QIIME pipelin...

The concentration and isotopic composition of uranium (δ238U, 234U/238U activity ratio) in combination with traditional isotopes (δ18O, δ13C) were examined as potential tracers authigenic carbonate formation a karst aquifer. U ratios the tufa-precipitating sections two connected rivers (Krka Zrmanja, Croatia) decreased downstream water precipitated due to active self-purification processes, i.e...

The elongation factor Tu, encoded by tuf genes, is a GTP binding protein that plays a central role in protein synthesis. One to three tuf genes per genome are present, depending on the bacterial species. Most low-G+C-content gram-positive bacteria carry only one tuf gene. We have designed degenerate PCR primers derived from consensus sequences of the tuf gene to amplify partial tuf sequences fr...

Potensi air tanah sangat dipengaruhi kondisi geologi dan hidrogeologi, sehingga sebaran lapisan akuifer beragam dalam satu wilayah. Hal ini terjadi di wilayah Kecamatan Pakis Kabupaten Malang. Beberapa masih mengalami kesulitan memanfaatkan sebagai sumber bersih. Penelitian bertujuan untuk mengidentifikasi memetakan dangkal Pakis. Untuk menyelesaikan penelitian, dilakukan pengukuran dengan meto...

Introduction The deterioration process of inorganic materials induced by microorganisms, including glass, marble, concrete, sandstone, tufa, and metals, can cause various alterations such as crust formation on surfaces, change of hue, discoloration, and loss of materials, leading to structural damage (Figure 1). These severe problems are due to the excretion of aggressive metabolic products suc...