Thymidylate synthase (TS) gene expression is modulated by a polymorphism in the 5' regulatory region of the gene. The polymorphism consists mainly of either two repeats (2R) or three repeats (3R) of a 28-bp sequence, yielding greater TS gene expression and protein levels with a 3R genotype. Two USF family E-box consensus elements are found within the tandem repeats of the 3R genotype, and one i...

This study demonstrates the potential of the oleaginous fungus Mucor circinelloides for synthesizing oil rich in polyunsaturated fatty acids when cultivated in thin stillage (TS) and centrifuged thin stillage (CTS) from corn-to-ethanol production. Biomass yield, oil yield and its EPA content were studied at different cultivation periods and carbon to nitrogen ratios. The fungal biomass yield (g...

The purpose of this report is to highlight the utility of prenatal MRI as an adjunctive imaging modality in the diagnosis and prognosis of Tuberous Sclerosis (TS) (Bourneville's disease). We report a case of TS detected in utero at 30 weeks gestation. A routine ultrasonography at 26 weeks in a 28-year-old primigravida was followed by an ultrafast MRI examination at 30 weeks gestation. Ultrasoun...

miR-28-5p is an intragenic miRNA which is underexpressed in several tumor types showing a tumor suppressor (TS) activity. Routinely, the known miR-28-5p targets are validated in specific tumor contexts but it is unclear whether these targets are also being regulated in other tumor types. To this end, we adopted the miRNA pull out assay to capture the miR-28-5p targets in DU-145 prostate cancer ...

Synthetic genes based on deduced amino acid sequences of the NAD-dependent DNA ligase (ligA) and CTP synthetase (pyrG) of psychrophilic bacteria were substituted for their native homologues in the genome of Salmonella enterica serovar Enteritidis phage type 13a (PT13a). The resulting strains were rendered temperature sensitive (TS) and did not revert to temperature resistance at a detectable le...

Weighted mean temperature of the atmosphere, Tm, is a key parameter for retrieving precipitable water vapor from Global Navigation Satellite System observations. It commonly estimated by linear model that relates to surface Ts. However, relationship between Tm and Ts associated with geographic regions affected weather. To better estimate over China, we analyzed region-specific weather-dependent...

Because infection and immune responses have been implicated in the pathogenesis of Tourette syndrome (TS), we hypothesized that children with TS would have altered gene expression in blood compared to controls. In addition, because TS symptoms in childhood vary with age, we tested whether gene expression changes that occur with age in TS differ from normal control children. Whole blood was obta...

Recently, we have demonstrated that thymidylate synthase (TS) protein expression predicts for the clinical response to a regimen of infusional 5-fluorouracil (5FU) in advanced colorectal cancer patients. Previous studies by other groups that showed a correlation between TS gene expression and response to the fluoropyrimidine also involved infusional regimens. Considering the putatively differen...

Breast cancer (BC) is the leading cause of cancer-related deaths among women in Mexico. Two single-nucleotide polymorphisms (SNPs) in the thymidylate synthase (TS) gene, the 28-base pair (bp) tandem repeat in the TS 5'-untranslated enhanced region (TSER) and the 6-bp insertion/deletion in the TS 3'-untranslated region (TS 3'-UTR), increase the rate of misincorporation of uridylate into DNA and ...

Klíčová slova kardiální autonomní funkce – turbulence srdeční frekvence – migréna Abstract Studies of autonomic nervous system function in patients with migraine have shown conflicting results. Heart rate turbulence (HRT) is a new method to assess cardiac autonomic function. HRT impairment reflects cardiac autonomic dysfunction. The aim of the present study was to determine validation of HRT as...