Previous studies have indicated that in all land plants examined to date, the chloroplast gene trnLUAA isinterrupted by a single group I intron ranging from 250 to over 1400 bp. The parasitic Epifagus virginiana haslost, however, the entire gene. We report that the intron is missing from the chloroplast genome of twoarctic species of the legume genus Hedysarum (H. alpinum, H. ...

Premise The objective of this study was to optimize an existing DNA extraction protocol for recalcitrant plant taxa obtain high-quality from preserved herbarium tissue suitable downstream PCR applications. Methods and Results Leaf 30 diverse species obtained the U.S. National Arboretum Herbarium. Our previous (Gouker et al., 2020, Applications in Plant Sciences 8: e11403) improved by use 10X Tr...

DNA barcoding should provide rapid, accurate and automatable species identifications by using a standardized DNA region as a tag. Based on sequences available in GenBank and sequences produced for this study, we evaluated the resolution power of the whole chloroplast trn L (UAA) intron (254-767 bp) and of a shorter fragment of this intron (the P6 loop, 10-143 bp) amplified with highly conserved...

Overall phylogenetic relationships within the genus Pelargonium (Geraniaceae) were inferred based on DNA sequences from mitochondrial(mt)-encoded nad1 b/c exons and from chloroplast(cp)-encoded trnL (UAA) 59 exon–trnF (GAA) exon regions using two species of Geranium and Sarcocaulon vanderetiae as outgroups. The group II intron between nad1 exons b and c was found to be absent from the Pelargoni...

Overall phylogenetic relationships within the genus Pelargonium (Geraniaceae) were inferred based on DNA sequences from mitochondrial(mt)-encoded nad1 b/c exons and from chloroplast(cp)-encoded trnL (UAA) 5' exon-trnF (GAA) exon regions using two species of Geranium and Sarcocaulon vanderetiae as outgroups. The group II intron between nad1 exons b and c was found to be absent from the Pelargoni...

Pachybrachis holerorum n. sp. is described from the northern Apennines, Italy. The new species is related to P. karamani (Weise, 1893), from which it differs in the shape of the median lobe of the aedeagus and in small differences in chromatic pattern. The close relationship with P. karamani is confirmed by molecular analyses performed on a fragment of 829 nucleotides of the mitochondrial gene ...

Noncoding DNA sequences from numerous regions of the chloroplast genome have provided a significant source of characters for phylogenetic studies in seed plants. In lycophytes and monilophytes (leptosporangiate ferns, eusporangiate ferns, Psilotaceae, and Equisetaceae), on the other hand, relatively few noncoding chloroplast DNA regions have been explored. We screened 30 lycophyte and monilophy...

Disentangling the effects of glaciers and climate on vegetation is complicated by confounding role that plays in both systems. We reconstructed changes occurring over Holocene at Jøkelvatnet, a lake located directly downstream from Langfjordjøkel glacier northern Norway. used sedimentary ancient DNA (sedaDNA) metabarcoding dataset 38 samples sediment core spanning 10,400 years using primers tar...

Little is currently known about preservation of plant DNA in lake sediments. Most prior information originates from laboratory experiments while systematic field-based studies are still lacking. Here, we used the “g” and “h” universal primers for P6 loop region chloroplast trnL (UAA) intron to amplify 219 surface sediments China Siberia. We introduce (i) percentage sequence counts with best ide...