A comparative evaluation of four conservation chemicals (96% ethanol, 1% sodium benzoate, 10% formalin and glycerol), used to preserve Trichinella spiralis larvae's over 120 days, was performed. The results obtained after conservation with 96% ethanol, 1% sodium benzoate and glycerol showed no statistically significant differences in both the shape and the diameter in coiled Trichinella spirali...

Detection of the repetitive sequence pPra specific for Trichinella spiralis was carried out by PCR in the blood of 37 patients infected tout to six weeks previously, during a horse-meat related outbreak of trichinellosis (Paris, 1993). Only two patients of 37 wete PCR-positive. Human blood was sampled during the febrile phase of the disease, before any antihelminthic treatment. To assess the va...

North American genotypes of Trichinella spiralis (T-1), Trichinella nativa (T-2), Trichinella pseudospiralis (T-4), Trichinella murrelli (T-5), and Trichinella T-6 were examined for susceptibility to freezing in pork using time-temperature combinations that have been proven to inactivate T. spiralis. Infections were established in 3-month-old pigs of mixed sex and breed by oral inoculation of 1...

Cell-mediated immunity during the muscular phase of Trichinella infection in humans was studied. Cell proliferation, the phenotypic changes in the T-cell population, and expression and production of cytokines were examined by using peripheral blood mononuclear cells (PBMC) collected at different times postinfection from 10 individuals who had acquired Trichinella spiralis and five individuals w...

Analysis of taxonomic affiliation of Trichinella species circulating in the Chukotka Autonomous Region and some subarctic areas of the Russian Federation showed that the representatives of T. spiralis and the Arctic trichinellas T. nativa (genotype T2) and Trichinella sp. (genotype T6) can be found there. The partial sequences of Coxb (704 bp) of these Arctic Trichinella spp. from Russia differ...

A Trichinella spiralis isolate obtained from a gray wolf (Canis lupus) in northwestern Montana in 1987 was evaluated for infectivity and resistance to freezing and its DNA restriction fragment length polymorphisms (RFLP's) compared to that from other sylvatic and domestic isolates. Infectivity indices in albino mice were low (<2.6) and correlated well with mouse infectivity data from other sylv...