This study investigates the ability of lymphocytes to utilize fatty acids originating from triacylglycerols and the effect of triacylglycerols upon mitogen-stimulated lymphocyte proliferation. Lymphocytes isolated from rat lymph nodes, spleen, thymus and lymphatic duct had a lipoprotein lipase activity of approx. 10 units/mg of protein, indicating that the fatty acids of circulating triacylglyc...

The synthesis of structured lipids with nutraceutical applications, such as medium-long-medium (MLM) triacylglycerols, via modification oils and fats represents a challenge for the food industry. This study aimed to synthesize MLM-type dietary triacylglycerols by enzymatic acidolysis cottonseed oil capric acid (C10) catalyzed Lipozyme RM IM (lipase from Rhizomucor miehei) in fluidized bed react...

Dietary fats are converted into chylomicron triacylglycerols via the 2-monoacylglycerol and phosphatidic acid pathways of acylglycerol formation. In view of the known positional and fatty acid specificity of the acyltransferases, the triacylglycerol structures resulting from the two pathways would be expected to differ, but this has not been demonstrated. We have performed stereospecific analys...

To explore the hypothesis that proliferating lymphoid cells in immune-stimulated lymph nodes obtain nutrients locally from adjacent adipose tissue, adult guinea pigs were fed for 6 weeks on standard chow or on chow supplemented with 100 g suet, sunflower oil or fish oil/kg. All the guinea pigs ate standard chow for the last 5 d, during which swelling of one popliteal lymph node was stimulated b...

The resolution of molecular species of triacylglycerols by reversed-phase high-performance liquid chromatography is discussed. Particular attention is given to the choice of stationary phase, mobile phase, injection solvent, detector and the effect of temperature. The influence of molecular structure on retention time is also explained. Triacylglycerols are major components of the human diet, s...

FCHL (familial combined hyperlipidaemia) is a highly prevalent genetic lipid disorder that accounts for a substantial number of premature cardiovascular events. To date, FCHL has been complicated by the different lipid phenotypes that are present within one family and one individual patient over time. In the present study, we hypothesized that a parabolic relationship between plasma triacylglyc...

Thlaspi arvense L. (pennycress) is a cold-tolerant Brassicaceae that produces large amounts of seeds rich in triacylglycerols and protein, making it an attractive target for domestication into offseason oilseed cash cover crop. Pennycress easily genetically transformed, enabling synthetic biology approaches to tailor oil properties specific biofuel industrial applications. To test the feasibili...

In Western countries, the transport of triacylglycerols by plasma triacylglycerol-rich lipoproteins (chylomicra and very-low-density lipoproteins (VLDL)) accounts for approximately 100 g lipids/d and represents a major pathway in the delivery of energy to tissues. Fat emulsions have been developed to resemble endogenous chylomicra (Wretlind, 1981). However, if the particle size of emulsion drop...

of dietary medium-chai" triacylglycerols (MCTs) on serum lipid levels, liver function, and hepatic fat accumulations in healthy men. Eleyen subjects consumed 2200-2600 kcal daily, of which 70-80 g was fat; the fat included 40 g of MCTs or else 40 g of long-chain triacylglycerols (blended yegetable oil). The diet was followed for 4 weeks in this controlled double-blind study. At the end of the e...

The new photometric lipase determinations use diacylglycerols as substrates in a micellar solution, whereas the turbidimetric method is based on emulsified triacylglycerols degradation. Extrahepatic lipoprotein lipase, which is well known to be liberated by heparin from the capillar binding sites, may cleave triacylglycerols as well as diacylglycerols. When a heparin bolus injection is given to...