Trs20p is a subunit of the evolutionarily conserved TRAPP (TRAnsport Protein Particle) complex that mediates various aspects of membrane trafficking. Three TRAPP complexes have been identified in yeast with roles in ER-to-Golgi trafficking, post-Golgi and endosomal-to-Golgi transport and in autophagy. The role of Trs20p, which is essential for viability and a component of all three complexes, a...

Bet3p, a component of a large novel complex called TRAPP, acts upstream of endoplasmic reticulum (ER)-Golgi SNAREs. Unlike the SNAREs, which reside on multiple compartments, Bet3p is localized exclusively to Golgi membranes. While other proteins recycle from the Golgi to the ER, Bet3p and other TRAPP subunits remain associated with this membrane under conditions that block anterograde traffic. ...

BACKGROUND The transport of endoplasmic reticulum (ER)-derived COPII vesicles toward the ER-Golgi intermediate compartment (ERGIC) requires cytoplasmic dynein and is dependent on microtubules. p150(Glued), a subunit of dynactin, has been implicated in the transport of COPII vesicles via its interaction with COPII coat components Sec23 and Sec24. However, whether and how COPII vesicle tether, TR...

Billions of packets traverse computer networks every day. Often, these packets have legitimate destinations such as buying a book at amazon.com or streaming a video. Unfortunately, malicious and suspicious network traffic continues to plague the Internet. One example is abusing the Domain Name System (DNS) protocol to exfiltrate sensitive data, establish backdoor tunnels, or control botnets. To...

The TRAPP (transport protein particle) complexes are tethering complexes that have an important role at the different steps of vesicle transport. Recently, the crystal structures of the TRAPP subunits SEDL and BET3 have been determined, and we present here the 1.7 Angstroms crystal structure of human TPC6, a third TRAPP subunit. The protein adopts an alpha/beta-plait topology and forms a dimer....

Strict consistency of replicated data is infeasible or not required by many distributed applications, so current systems often permit stale replication, in which cached copies of data values are allowed to become out of date. Queries over cached data return an answer quickly, but the stale answer may be unboundedly imprecise. Alternatively, queries over remote master data return a precise answe...

Billions of legitimate packets traverse computer networks every day. Unfortunately, malicious traffic also traverses these same networks. An example is traffic that abuses the Domain Name System (DNS) protocol to exfiltrate sensitive data, establish backdoor tunnels or control botnets. This paper describes the TRAPP-2 system, an extended version of the Tracking and Analysis for Peer-to-Peer (TR...

Intracellular vesicle transport is a mechanism required for the proper targeting and secretion of proteins and lipids. The process comprises sorting, budding, movement, tethering and fusion of the vesicle with the target membrane resulting in delivery of its contents [1]. Transport protein particle (TRAPP) has been demonstrated to contribute to endoplasmic reticulum (ER)-to–Golgi, intra-Golgi a...

Transport protein particle (TRAPP) I is a multisubunit vesicle tethering factor composed of seven subunits involved in ER-to-Golgi trafficking. The functional mechanism of the complex and how the subunits interact to form a functional unit are unknown. Here, we have used a multidisciplinary approach that includes X-ray crystallography, electron microscopy, biochemistry, and yeast genetics to el...

The TRAPP complex identified in yeast regulates vesicular transport in the early secretory pathway. Although some components of the TRAPP complex are structurally conserved in mammalian cells, the function of the mammalian components has not been examined. We describe our biochemical and functional analysis of mammalian Bet3, the most conserved component of the TRAPP complex. Bet3 mRNA is ubiqu...