salinity is a major limiting factor for plant growth and development. to evaluate the impact of p5cs gene expression under in vitro salt stress condition, transgenic tobacco (nicotiana plumbaginifolia) carrying p5cs gene and non-transgenic plants were treated with 0, 100, 150, 200 or 250 mm nacl for 28 days. proline content, lipid peroxidation and the activity of some antioxidant enzymes after ...

Transformation of plants using Agrabacterium rhizogenes may affect secondary metabolite production as well as morphological changes. In this study, T-DNA from Ri plasmid in A. rhizogenes carrying pRi15834-PRT35S-GUS was introduced into tobacco leaf segments to initiate development of transformed hairy roots. Plant regeneration from transgenic roots used MS medium, and plants regenerated fro...

The main aim of this study was to obtain the Crimean-Congo hemorrhagic fever virus (CCHFV) glycoprotein, through either stable transgenic plants or using a transient expression system, and determine the yield, quality and finally the immunogenicity of the plant-made CCHFV glycoprotein in a mouse model. We designed and synthesized a codon-optimized G1/G2 gene from the G1 and G2 parts of the CCHF...

a significant portion of the world’s cultivated land is affected by salinity that reduces crop productivity in these areas. breeding for salt tolerance is one of the important strategies to overcome this problem. recently, genetic engineering is becoming a promising approach to improving salt tolerance. in order to improve the yield performance of canola in saline soils, we transformed canola w...

background: nicotiana tobacco contains large amounts of alkaloid nicotine. tobacco plant is used for smoking and causes many health problems since it is high in nicotine which is one of the widely-recognized toxic compounds with serious side effects for different body organs. reducing nicotine content of this plant is a way to reduce its health hazards in cigarette smokers. utilizing the new me...

plant tissue culture techniques are used as basic requirement of common plant transformation systems. in most cases of plant transformation, a reproducible regeneration protocol is the limiting step due to long time lasting, specialized facilities and well experienced persons. furthermore, tissue culture procedures induce somaclonal variation among regenerated transgenic plants. therefore, rece...

To investigate the impact of overexpression of AtNRT2.1 transgene from Arabidopsis on nitrate uptake rate and to understand the regulation of endogenous HATS by nitrate and glutamine amino acid (Gln) in tobacco plants, wild-type and transgenic (F line) plants grown on soil for 4 weeks were transferred to hydroponic culture in a controlled-environment with a 16/8h L:D photoperiod at 24? C/20...

during plant infection, fungal pathogens secret polygalacturonase (pg) that are capable of degrading cell wall of susceptible plant tissues. polygalacturonase-inhibiting proteins (pgips) are able to specifically inhibit fungal polygalacturonases (pgs) activity. the inhibition of fungal pgs by pgips suggests that pgips have a role in plant tolerance to fungal infections. in this study bean (dane...