transformation of plants using agrabacterium rhizogenes may affect secondary metabolite production as well as morphological changes. in this study, t-dna from ri plasmid in a. rhizogenes carrying pri15834-prt35s-gus was introduced into tobacco leaf segments to initiate development of transformed hairy roots. plant regeneration from transgenic roots used ms medium, and plants regenerated fro...

Cotton cultivar Coker has been already transformed with recombinant pBI121-chi via Agrobacterium tumefaciens. The T-DNA region of pBI121-chi carries the chitinase (chi ) gene from bean and is under the control of the CaMV35S promoter. T1 and T2 progenies of transgenic cotton containing the chi gene were used in this study.  Polymerase chain reaction (PCR), Southern and Western blotting data con...

Background: A highly efficient genetic transformation system is essential for a successful genetic manipulation of the African violet (Saintpaulia ionantha Wendl.). Objectives: Developing a particle bombardment-based genetic transformation system for the African violet. Materials and Methods: A local cultivar of the African violet from Guilan province was used for transformation experiments. Th...

pod borer is one of the main causes for yield loss of chickpea. therefore, breeding of chickpea for resistance to this pest is important. the use of cry toxin from bacillus thuringiensis is an effective strategy for producing of transgenic resistant chickpea to this pest. these toxins are able to become active in the midgut of larvae and disrupt the insect's digestive system. we studied the sta...

Abstract Background: Selectable marker gene (SMG) systems are critical for generation of transgenic crops. Transgenic crop production Background: Selectable marker gene (SMG) systems are critical for generation of transgenic crops. Transgenic crop production without using SMG is not economically feasible. However, SMGs are non-essential once an intact transgenic plant has been established. Eli...

Background: Canola is an agro-economically oilseed crop. Yield loss due to fungal disease of stem rot caused by Sclerotinia sclerotiorum is a serious problem in canola cultivation. Thaumatin-like proteins are large groups of the pathogenesis-related proteins which provide resistance to the fungal infection in response to invading pathogens and play a key role in plant defense s...

low phosphorous (p) availability in soils limits production of soybean [glycine max (l.) merr.] around the world. this study was conducted to determine whether exogenous expression of the rice (oryza sativa l.) phosphates transporter gene ospt2 would increase inorganic phosphates (pi) acquisition and improve yield in transgenic soybean. cotyledonary-node explants of the soybean were inoculated ...