Experiments examining potential impacts of growth hormone (GH) transgenesis in fish typically use a single source strain and do not address differential strains different genetic backgrounds. Here, we examine the effects differing backgrounds when reared culture on transgenic nontransgenic coho salmon (Oncorhynchus kisutch) produced by mating sires from rivers with dams origin. We found signifi...

Many immuno-therapeutic strategies are currently being developed to fight cancer. In this scenario, oncolytic adenoviruses (Onc.Ads) have an interesting role for their peculiar tumor selectivity, safety, and transgene-delivery capability. The major strength of the Onc.Ads is extraordinary immunogenicity that leads a strong T-cell response, which, together with possibility delivery therapeutic t...

The ability to conduct reverse genetic studies in symbiotic systems is enabled by transgene expression and transformation of at least one partner. relationship between the yellow spotted salamander, Ambystoma maculatum, green alga, Oophila amblystomatis, a unique model vertebrate-algae symbiosis. Despite over 130 years scientific study, there are still many open questions this Transgene partner...

transgene integration and expression in host plant is quite unpredictable and is considered as the major problem in plant transformation. the variation in transgene copy number in transgenic plants influences the expression level and is one of such complication. in many plant species, the analysis of transgenic plants has shown that independent transgenic plants have one to many copies of trans...

abstract background: determination of transgenic embryos from non transgenic embryos sibling is an important step in producing homozygous transgenic mice. these steps need by pcr or southern blotting followed extraction of dna, but both techniques require skill and consume time. objective: the aim of this study was simulation of high accuracy method using novel enhanced green fluorescent protei...

The recombinant adeno-associated viral (rAAV) vector is one of the most effective vectors in gene therapy because its low immunogenicity, high transduction efficiency, broad tissue specificity, and long-term transgene expression ability. HEK293T cells are widely used to produce rAAV vectors, acquisition rAAV-producing essential processes for higher production. To acquire such cells, a method id...