The transition from normal forage to a highly fermentable diet to achieve rapid weight gain in the cattle industry can induce ruminal acidosis. The molecular host mechanisms that occur in acidosis are largely unknown. Therefore, the histology and transcriptome profiling of rumen epithelium was investigated in normal and acidosis animals to understand the molecular mechanisms involved in the dis...

The transcriptome is the mRNA transcript pool in a cell, organ or tissue with the liver transcriptome being amongst the most complex of any organ. Functional genomics methodologies are now being widely utilized to study transcriptomes including the hepatic transcriptome. This review outlines commonly used methods of transcriptome analysis, especially gene array analysis, focusing on publication...

BACKGROUND Current standard operating procedures for salivary transcriptomic analysis require low temperatures and lengthy mRNA isolation, which substantially hamper its use in the clinic. We developed a streamlined, ambient-temperature processing, stabilization, and storage protocol for clinical analysis of salivary RNA. METHODS The direct saliva transcriptome analysis (DSTA) used cell-free ...

Platelets play a critical role in hemostasis and are involved in wound repair and inflammatory processes. Contact to the subendothelial matrix induces platelet activation that is followed by release of cytokines and growth factors and formation of a vascular plug. Several inherited defects of platelet function resulting in bleeding diathesis are known. However, only in the minority of these dis...

Expression of approximately two-thirds of the vaccinia virus (VACV) genome has been previously analyzed gene by gene, and the analysis has led to the classification of early-, intermediate-, and late-stage genes with distinct promoters and associated transcription factors. Assarsson et al. (1) reported the complete VACV transcriptome by using a tiling array. There are problems in application of...