PURPOSE Hyaluronan (HA) is a high-molecular weight glycosaminoglycan that can affect water and solute fluxes in the extracellular matrix. The distribution of HA in the human trabecular meshwork of nonglaucomatous eyes was examined to help understand the potential role of HA in the regulation of aqueous outflow resistance. METHODS Histolocalization of HA was established in situ in the trabecul...

Selective laser trabeculoplasty (SLT) is a relatively recent modality for treating patients with open-angle glaucoma and ocular hypertension. Similarly to argon laser trabeculoplasty, SLT uses laser energy to target the trabecular meshwork and lower intraocular pressure by increasing aqueous outflow. However, although argon laser trabeculoplasty has been used successfully for several years to t...

PURPOSE To characterize cyclic nucleotide phosphodiesterase isozyme activities in human trabecular meshwork cells and primary cultures of porcine trabecular meshwork cells. METHODS Radioimmunoassay of acetylated acid extracts was used to determine changes in cyclic adenosine monophosphate (cAMP) and cyclic quanosine monophosphate (cGMP) in human trabecular meshwork cells treated with phosphod...

In this article we present computational results for the pressure in the human eye. Pressure computations for different flow rates of the aqueous humor, viscosity of the aqueous humor, and permeability of the trabecular meshwork are given. The fluid flow is assumed to be axisymmetric, and modeled as a coupled systems of Stokes and Darcy fluid flow equations, represented the fluid flow in the an...

purpose : to investigate the effects of mtp-131 on trabeculum cells cultured under hydrostatic pressure methods : glaucomatous human trabecular meshwork (gtm3) cells were divided into three groups: the mtp-131 group, the hydrostatic group, and the normal group. the mtp-131 group was pretreated with 1 µm mtp-131, and the cells were then cultured under 80 mmhg pressure for 24 hours. the normal gr...

OBJECTIVE To determine the effects of H-7 (1-[5-isoquinoline sulfonyl]-2-methyl piperazine) on the structure and fluid conductance of the trabecular meshwork of live cynomolgus monkeys. METHODS Fluid outflow was measured by constant pressure perfusion of the anterior chamber with cationized and noncationized gold solution with or without H-7 in opposite eyes. The eyes were fixed by infusing I...

We examined a series of changes that occur in the trabecular meshwork fibers of human eyes during fetal development at 12-30 weeks of gestation. At 12 and 15 weeks, the uveal meshwork was stained black with silver impregnation (indicating the predominance of collagen types III and IV) in the endomysium of the ciliary muscle. At 20 weeks, in combination with Schlemm's canal, a dense fibrous tiss...

The in vivo response to short-pulsed Nd-YAG laser damage to the trabecular meshwork has not been studied in the human eye. The nature of the response will determine the potential efficacy of this treatment for glaucoma. We have investigated short-pulsed laser trabeculotomy lesions created in the trabecular meshwork of four human eyes within 18 hr prior to enucleation for intraocular melanoma. S...

BACKGROUND Primary open-angle glaucoma (POAG) is the most common form of glaucoma which is an irreversible blind leading disease and lacks effective remedies. In recent years, POAG has been linked to the gene MYOC encoding myocilin that has been identified to harbor causal mutations. A variety of studies show that the mutant myocilin acts by gain of function. The mutant MYOC protein induces end...