Potato protein liquor (PPL), a side stream from the potato starch industry, is normally used as fertilizer. However, with more than 100 g/L of sugars, 20 g/L of Kjeldahl nitrogen and Chemical Oxigen Demand (COD) of 300 g/L, it represents serious environmental challenges. The use of PPL for fungal cultivation is a promising solution to convert this waste into valuable products. In this study, PP...

The protein content of dry biomass of the microalgae Porphyridium cruentum, Scenedesmus almeriensis, and Muriellopsis sp. and of the cyanobacteria Synechocystis aquatilis and Arthrospira platensis was measured by the Lowry method following disruption of the cells by milling with inert ceramic particles. The measurements were compared with the Kjeldahl method and by elemental analysis. The nitro...

The micro-Kjeldahl method for nitrogen determination has been widely used in organic chemistry laboratories in recent years. The value of this method, developed through the brilliant researches of PiLcH, BANG, PARNAS and WAGNER, PREGL, and coworkers, has justified its application to industrial and clinical problems. Plant physiologists have not generally adopted quantitative micro-methods for n...

The increase in total Kjeldahl nitrogen (TKN) concentrations is correlated with increases cyanobacterial bloom biomass. Standard methods for the measurement of TKN are tedious, costly, time-consuming and involve use hazardous catalysts, such as mercury, high temperatures significant amounts toxic acids bases. Since plays a pivotal role influencing algal blooms, there an urgent need to develop s...

J. Inst. Brew. 115(2), 122–127, 2009 In this study we present a ninhydrin based microwell assay that can be utilized in place of the traditional Kjeldahl method for the determination of the protein content of beer or wine. In addition, the assay is ideal for the determination of free amino acids in beer (FAN), a term understood and used by brewers, and yeast assimilable nitrogen (YAN) used by e...

A comparative study was conducted between exotic and local (epigeic--Eisenia fetida and anaecic--Lempito mauritii, respectively) species of earthworms for the evaluation of their efficacy in vermicomposting of municipal solid waste (MSW). Vermicomposting of MSW for 42 days resulted in significant difference between the two species in their performance measured as loss in total organic carbon, c...

Abbreviations: BOD: biochemical oxygen demand COD: chemical oxygen demand OD: optical density OW: optimized wastewater PNSB: purple non sulphur photosynthetic bacteria ROW:raw optimized wastewater RW: raw wastewater SCP: single cell protein SEM: scanning electron microscope SOW: sterile optimized wastewater SW: sterile wastewater TEM: transmission electron microscope TKN: total kjeldahl nitroge...

When poultry manure is collected but cannot be analyzed immediately, a method for storing the manure is needed to ensure accurate subsequent analyses. This study has 3 objectives: (1) to investigate effects of 4 poultry manure sample preservation methods (refrigeration, freezing, acidification, and freeze-drying) on the compositional characteristics of poultry manure; (2) to determine compositi...

We evaluated four spectrophotometric methods for suitability in determining total protein in human milk samples. Results were compared with those by an established method for the determination of total nitrogen (micro-Kjeldahl). Values for total protein by all four methods significantly correlated with micro-Kjeldahl. For the biuret assay, Lowry-Peterson assay, Bio-Rad Coomassie Blue assay, and...