نتایج جستجو برای: tof and esi

تعداد نتایج: 16829797  

Journal: :Journal of biomolecular techniques : JBT 2007
Yong Yang Sheng Zhang Kevin Howe David B Wilson Felix Moser Diana Irwin Theodore W Thannhauser

The use of nLC-ESI-MS/MS in shotgun proteomics experiments and GeLC-MS/MS analysis is well accepted and routinely available in most proteomics laboratories. However, the same cannot be said for nLC-MALDI MS/MS, which has yet to experience such widespread acceptance, despite the fact that the MALDI technology offers several critical advantages over ESI. As an illustration, in an analysis of mode...

Journal: :Journal of chromatography. A 2006
Hauke Hilz Laura E de Jong Mirjam A Kabel Henk A Schols Alphons G J Voragen

Different separation (HPAEC, RP-HPLC, CE) and identification (MALDI-TOF-MS, ESI-MS(n)) techniques were compared to analyse oligosaccharides obtained after incubation of xyloglucan with endo-glucanase. It was possible to analyse xyloglucan oligosaccharides with each technique. Several techniques, including off line (HPAEC-MALDI-TOF-MS) or online (CE-ESI-MS(n), RP-HPLC-ESI-MS(n)) connection provi...

2014
Li-Ping Bai Jie Liu Li Han Hing-Man Ho Renxiao Wang Zhi-Hong Jiang

A comparative study on human telomeric DNA G-quadruplex binding of meso-5,10,15,20-tetrakis(N-methyl-4-pyridyl)porphyrin (TMPyP4) between its two salt forms, i.e., tetratosylate and tetrachloride, was conducted by using ESI-TOF-MS, UV-melting measurement, and molecular modeling methods. Besides cation TMPyP4, the tosyl anion was found to bind to human telomeric DNA G-quadruplex with multiple bi...

Journal: :Methods in enzymology 1996
J C Schwartz I Jardine

A number of other features of ITMS systems that will enhance their ability to analyze biological macromolecules are worth mentioning. As has already been demonstrated for ESI/quadrupole, ESI/magnetic sector, and ESI/FTICR systems, the capability of inducing fragmentation of the ESI-generated multiply charged ions of biological macromolecules in the capillary/skimmer region of the ESI source and...

Journal: :Molecules 2013
Heng-Qiang Zhao Xiao Wang Hong-Mei Li Bin Yang Hong-Jun Yang Luqi Huang

A method combining hydrophilic interaction chromatography (HILIC) and electrospray ionization mass spectrometry (ESI-MS) was developed for the characterization and determination of natural Cordyceps. Separation was achieved on a Waters Xbridge Amide column with gradient elution. Identification of 15 target nucleosides and nucleobases was based on retention time, UV spectra and mass measurements...

2016
Yan-Xin Zhang Xin Yang Pan Zou Peng-Fei Du Jing Wang Fen Jin Mao-Jun Jin Yong-Xin She

The HPLC system was equipped with a Waters XBridgeTM C18 column (2.1 × 150 mm, 5 μm), and the column temperature was set to 25 °C. The mobile phases for metabolic fingerprinting consisted of 0.1% formic acid in Milli-Q water and 5 mM ammonium acetate in Milli-Q water (solvent A, positive electrospray ionization (ESI+) and negative electrospray ionization (ESI–), respectively), acetonitrile (sol...

Journal: :Journal of proteomics 2012
Erica Gianazza Clizia Chinello Veronica Mainini Marta Cazzaniga Valeria Squeo Giancarlo Albo Stefano Signorini Salvatore S Di Pierro Stefano Ferrero Simone Nicolardi Yuri E M van der Burgt André M Deelder Fulvio Magni

Renal cell carcinoma (RCC) is typically asymptomatic and surgery usually increases patient's life only for early stage tumors. However, some cystic and solid renal lesions cannot be confidently differentiated from clear-cell-RCC. Therefore possible markers for early detection and to distinguish malignant kidney tumors are needed. To this aim, we applied MALDI-TOF and LC-MS/MS analysis to RPC18 ...

2014
Li-Ping Bai Masaki Hagihara Kazuhiko Nakatani Zhi-Hong Jiang

A study on binding of antitumor chelerythrine to human telomeric DNA/RNA G-quadruplexes was performed by using DNA polymerase stop assay, UV-melting, ESI-TOF-MS, UV-Vis absorption spectrophotometry and fluorescent triazole orange displacement assay. Chelerythrine selectively binds to and stabilizes the K(+)-form hybrid-type human telomeric DNA G-quadruplex of biological significance, compared w...

2013
Esra Altuntas Anna Crecelius Ulrich S. Schubert

ESI-Q-TOF and MALDI-TOF/TOF mass spectrometers were utilized to elucidate the macromolecular structures of linear poly(ethylene imine) (PEI) polymers, which are of importance in gene delivery. A detailed characterization of linear PEIs was performed by tandem mass spectrometry (MS/MS). This study provides important information with regard to the fragmentation mechanism of PEI polymers and the c...

نمودار تعداد نتایج جستجو در هر سال

با کلیک روی نمودار نتایج را به سال انتشار فیلتر کنید