leaf and root explants from 10 to 20 day– old seedlings of plantago lanceolata, were cultured on ms, msh, nt and b5 basal media with various concentrations of different plant growth regulators. the best callus induction was obtained with 0.4 mgl-1 2,4-d in msh i with 400 callus index. callus growth was significantly stimulated (p=0.05)with 0.8 mgl-1 2,4-d and 0.1 mgl-1 kin in msh ii, with 7...

abstract gerbera is one of the most demanded plants in commercial cut flowers plants. optimizing the micro-propagation of new cultivars is therefore important. in this research “red explosion” cultivar was used and capitola (as explants) were directed to a modified solid murashigue and skoog medium with four concentration of tdz (0.25, 0.50, 0.75 and 1.00 mgl-1) for regeneration. three concentr...

Modification of the micro tissue culture technique, including incubation under increased CO(2), resulted in prolongation of the viability of the cells. As a consequence, satisfactory titrations of poliovirus and of poliovirus antiserum have been achieved by the micro method. The technique offers a number of advantages over conventional methods.

A rapid, potentially clinically useful test for detection of enterotoxigenic Escherichia coli is described. Whole bacterial cultures of enterotoxigenic E. coli, when briefly exposed to Y1 adrenal cells in tissue miniculture, effect a rounding response in the tissue culture that can be discerned at 18 to 24 h. The tissue culture technique agreed with the rabbit ileal loop in all 58 enterotoxigen...

given the importance of potato in the economy and people’s nutrition, as well as planting of disease-free seeds, it seems necessary to produce healthy seeds through tissue culture. this study was conducted during 2012-2013 at the research center of agricultural university of tajikistan aiming to evaluate the effect of different sizes of mini-tuber on yield and yield components of potato crop. a...

Background: Helicobacter pylori (H. pylori), is the major known infectious cause of gastric diseases in children and adults. The rate of antibiotic resistance to H. pylori treatment regimens has rapidly increased. We aimed to determine the sensitivity of helicobacter pylori in gastric tissue samples of children and adolescents to various antibiotics in Isfahan, Center of Iran. Materials and Met...

By means of a tissue culture technique, cells from chick embryos were procured in a state which proved to be suitable for electron microscopy. The electron micrographs disclosed details of cell structure not revealed by other methods of examination.