نتایج جستجو برای: time pplymerase chain reaction

تعداد نتایج: 2400757  

Journal: :iranian journal of virology 0
m salehi-vaziri department of virology and antimicrobial resistance research center, faculty of medicine, tehran university of medical sciences, tehran, iran sh monavari department of virology and antimicrobial resistance research center, faculty of medicine, tehran university of medical sciences, tehran, iran m khalili research and clinical center of infertility, yazd, iran m shamsi-shahrabadi department of virology and antimicrobial resistance research center, faculty of medicine, tehran university of medical sciences, tehran, iran h keyvani department of virology and antimicrobial resistance research center, faculty of medicine, tehran university of medical sciences, tehran, iran h mollaei department of virology and antimicrobial resistance research center, faculty of medicine, tehran university of medical sciences, tehran, iran

background and aims: sexually transmitted infections (stis) are of major concern to clinicians and researchers in the field of reproductive medicine. many sti pathogens cause incurable and often fatal diseases, and have been transmitted through insemination procedures. the role of herpes simplex virus in male infertility has been investigated using the sensitive methods. the aim of this study w...

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ژورنال: :کومش 0
حبیب جعفری نژاد habib jaafarinejad cancer research center and department of immunology, faculty of medicine, semnan university of medical sciences, semnan, iranمرکز تحقیقات سرطان، گروه ایمونولوژی، دانشکده پزشکی، دانشگاه علوم پزشکی سمنان، سمنان، ایران نیلوفر غنی زاده niloofar ghanizadeh cancer research center and department of immunology, faculty of medicine, semnan university of medical sciences, semnan, iranمرکز تحقیقات سرطان، گروه ایمونولوژی، دانشکده پزشکی، دانشگاه علوم پزشکی سمنان، سمنان، ایران فرحناز قهرمانفرد farahnaz ghahremanfard internal medicine department, semnan university of medical sciences, semnan, iranگروه پزشکی داخلی، دانشگاه علوم پزشکی سمنان، سمنان، ایران مهدی براتی mehdi barati cancer research center and department of immunology, faculty of medicine, semnan university of medical sciences, semnan, iranمرکز تحقیقات سرطان، گروه ایمونولوژی، دانشکده پزشکی، دانشگاه علوم پزشکی سمنان، سمنان، ایران احسان منوچهری ehsan manouchehri doulabi cancer research center and department of immunology, faculty of medicine, semnan university of medical sciences, semnan, iranمرکز تحقیقات سرطان، گروه ایمونولوژی، دانشکده پزشکی، دانشگاه علوم پزشکی سمنان، سمنان، ایران پرویز کوخایی parviz kokhaei immune and gene therapy lab, cancer centre karolinska, karolinska university hospital, stockholm, swedenآزمایشگاه ایمونولوژی و ژن درمانی، مرکز سرطان کارولینسکا، بیمارستان دانشگاه کارولینسکا، استکهلم، سوئد

هدف: لوسمی لنفوسیتی مزمن یکی از انواع سرطان های خون است که با تکثیر بیش ازحد سلول های b همراه می باشد. این بیماری به طور تقریبی در مردان بالای 50 سال شایع تر است . cll با نقص آپوپتوز در سلول های b همراه است. هدف ما در این مطالعه بررسی بیان پروتئین survivin به عنوان پروتئین مهارکننده آپوپتوز در سلول های تک هسته ای خون محیطی بیماران cll و افراد نرمال تحت تأثیر داروی بربرین در محیط in vitro است. م...

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Journal: :avicenna journal of medical biotechnology 0

background: dna isolation procedure can significantly influence the quantification of dna by real time pcr specially when cell free dna (cfdna) is the subject. to assess the extraction efficiency, linearity of the extraction yield, presence of co-purified inhibitors and to avoid problems with fragment size relevant to cfdna, development of appropriate external dna control (edc) is challenging. ...

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Journal: :jundishapur journal of microbiology 0
jila yavarian virology department, school of public health, tehran university of medical sciences, tehran, ir iran nastaran gavvami virology department, school of public health, tehran university of medical sciences, tehran, ir iran setareh mamishi pediatrics medical center, tehran university of medical sciences, tehran, ir iran; pediatrics medical center, tehran university of medical sciences, tehran, ir iran. tel: +98-2142933103, fax: +98-2188962343

conclusions according to this research and prevalence of hhv-6 in children, evaluation of csf (detecting the hhv-6 dna by pcr) is recommended in patients younger than 13 months with possible encephalitis. results from a total of 114 patients, hhv-6 was detected in 10 (8.8%), 90% of which were boys with mean age 7.7 months and median of 7.5 months. no significant differences were found in clinic...

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Journal: :hepatitis monthly 0
ahmet caliskan department of medical microbiology, necip fazil city hospital, kahramanmaras, turkey; medical microbiology laboratory, necip fazil city hospital, kahramanmaras, turkey. tel: +90-3442282800 ozlem kirisci department of medical microbiology, necip fazil city hospital, kahramanmaras, turkey esra ozkaya department of medical microbiology, necip fazil city hospital, kahramanmaras, turkey sevinc ozden department of medical microbiology, necip fazil city hospital, kahramanmaras, turkey seray tumer department of medical microbiology, necip fazil city hospital, kahramanmaras, turkey serkan caglar department of medical biohemistry, necip fazıl city hospital, kahramanmaras, turkey

conclusions in conclusion, the prevalence of hcv genotype 1 was 51.7%, which was lower than the rates reported in other studies in turkey, while the prevalence of hcv genotype 3 was 46%, which was remarkably higher than the reported turkish data. in addition, the prevalence rate for genotype 3 reported in the present study is the highest that has ever been reported in the literature. results th...

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Journal: :hepatitis monthly 0
hossein hadinedoushan immunology department, faculty of medicine, shahid sadoughi university of medical sciences, yazd, ir iran; immunology department, faculty of medicine, shahid sadoughi university of medical sciences, daneshjou blv, yazd, ir iran. tel: +98-3516233235, fax: +98-3516238561 hasan salmanroghani gastrology department, faculty of medicine, shahid sadoughi hospital, shahid sadoughi university of medical sciences, yazd, ir iran mohammad kazem amirbaigy gastrology department, faculty of medicine, shahid sadoughi hospital, shahid sadoughi university of medical sciences, yazd, ir iran mohsen akhondi-meybodi gastrology department, faculty of medicine, shahid sadoughi hospital, shahid sadoughi university of medical sciences, yazd, ir iran

conclusions findings of the present study showed that hcv genotype 3 was the predominant genotype followed by the subtypes 1a and 1b in yazd, central province of iran. in addition, there was no difference between hcv load and genotypes 1 and 3. hcv genotyping is recommended in other provinces of iran. objectives the main aim of this study was to estimate the prevalence of hcv genotypes in yazd,...

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Journal: :avicenna journal of medical biotechnology 0

background: discovery of short cell free fetal dna (cffdna) fragments in maternal plasma has created major changes in the field of prenatal diagnosis. the use of cffdna to set up noninvasive prenatal test is limited due to the low concentration of fetal dna in maternal plasma therefore, employing a high efficiency extraction method leads to more accurate results. the aim of this study was to ev...

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Journal: :archives of pediatric infectious diseases 0
daniela leite center of bacteriology, national reference laboratory for pertussis, instituto adolfo lutz, sao paulo, brazil; center of bacteriology, national reference laboratory for pertussis, instituto adolfo lutz. av. dr. arnaldo, 351 - 9ºandar, cep: 01246-902, sao paulo-sp, brazil. tel: +55-1130682896, fax: +55-1130819161 roberta morozetti blanco center of bacteriology, national reference laboratory for pertussis, instituto adolfo lutz, sao paulo, brazil leyva cecilia vieira de melo center of bacteriology, national reference laboratory for pertussis, instituto adolfo lutz, sao paulo, brazil cleiton eduardo fiorio center of bacteriology, national reference laboratory for pertussis, instituto adolfo lutz, sao paulo, brazil luciano moura martins center of bacteriology, national reference laboratory for pertussis, instituto adolfo lutz, sao paulo, brazil tania mara ibelli vaz center of bacteriology, national reference laboratory for pertussis, instituto adolfo lutz, sao paulo, brazil

background: bordetella pertussis is the causative agent of pertussis. in brazil, laboratory diagnosis of pertussis is based on the culture. in 2010, was standardized the real-time pcr taqman® in routine diagnosis. objectives: the aim of this study was to evaluate the impact achieved with the introduction of rt-pcr for the routine diagnosis of pertussis and to compare with the results obtained f...

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پایان نامه :وزارت علوم، تحقیقات و فناوری - دانشگاه پیام نور - دانشگاه پیام نور استان تهران - دانشکده علوم 1394
رمینا بارانی, غلامرضا مطلب, حسین مقصودی,

سرطان مری ec به عنوان یک دغدغه سلامت عمومی در سطح جهانی است، این سرطان با حدود 386000 مرگ ومیر در سال ششمین عامل مرگ ناشی از سرطان در جهان است، که حاصل مجموعه عامل های محیطی مانند دود تنباکو، رفلاکس معدی- مروی و تغییرات ژنتیکی است. inos بر اثر عوامل مختلف التهابی بیان می گردد و به همین دلیل نوع القایی nos نامیده می شود. تنظیم بیان آن در سطح ژن صورت گرفته ومیزان بیان آن ارتباط مستقیمی با تولید ب...

15 صفحه اول
پایان نامه :وزارت علوم، تحقیقات و فناوری - دانشگاه شهید چمران اهواز - دانشکده علوم پایه 1392
الهام پیشاور, محمد رمضانی, محمد شفیعی, خلیل آبنوس,

پلی اتیلن ایمین (pei) یک پلیمر پلی آمینی است که در مطالعات سلولی و بافتی به عنوان حامل غیر ویروسی کاربردهای موفقیت آمیزی داشته است. متاسفانه این ترکیب معایبی نیز دارد، از جمله اینکه منجر به سمیت سلولی می شود. اما مکانیسم های ایجاد سمیت آن هنوز به درستی شناخته نشده است. استفاده از تکنیک ترانسکریپتوم در شناسایی ژن های درگیر در فرایند آپوپتوزکمک شایانی به مشخص شدن مکانیسم سمیت این ترکیب می کند. ...

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