Three phase partitioning, a technique described for protein purification, has been employed for precipitation and purification of three different commercial preparations of alginates. Three phase partitioning works by the addition of /-butanol to aqueous solution of the polymer containing 20-30% ammonium sulphate (w/v). Three phases formed are: upper /-butanol layer, interfacial polymer precipi...

Three phase partitioning, a method generally used for protein separation, has been evaluated for extraction of oil from soybean. 82% oil was extracted within 1 h using this process which required simultaneous addition of t-butanol (1:1, v/v) and 30% ammonium sulphate to the soybean slurry.

Three phase partitioning (TPP) of starch and its derivatives was carried out by adding tert-butanol to the aqueous solution of the polysaccharides in presence of ammonium sulfate. Starch and its modified forms could be recovered in more than 90% yield in the form of an interfacial layer between aqueous and organic phases. Quantitative FT-IR spectral analysis of TPP-treated potato starch reveale...

The technique of three-phase partitioning (TPP) was used to purify the green fluorescent protein (GFP) in a single step. TPP uses a combination of ammonium sulphate and tert-butanol to precipitate proteins from their crude extracts. In the first round of TPP with 20% ammonium sulphate saturation at the ratio of crude to tert-butanol 1:1 (v/v), most of the GFP remains in the lower aqueous phase....

For outer-product–parallel sparse matrix-matrix multiplication (SpGEMM) of the form C=A×B, we propose three hypergraph models that achieve simultaneous partitioning of input and output matrices without any replication of input data. All three hypergraph models perform conformable one-dimensional (1D) columnwise and 1D rowwise partitioning of the input matrices A and B, respectively. The first h...

A study was conducted on the effect of two different biological factors, microbial surfactants and biodegradation, on the kinetics of partitioning of polycyclic aromatic hydrocarbons (PAHs) from nonaqueous-phase liquids (NAPLs). The effect of rhamnolipid biosurfactants on partitioning into the aqueous phase of naphthalene, fluorene, phenanthrene, and pyrene, initially dissolved in di-2-ethylhex...

in order to determine probable effects of sowing dates on partitioning coefficient of dry matter to different organs (leaf, stem and reproductive organs) in different wheat varieties (koohdasht, zagros and tajan) an experiment with crbd design and four replications was conducted. 5 sowing dates were included (29 december, 2007, 30 january, 2 march, 27 march, 1may, 2008). in order to quantify pa...