I T IS IJSUAL PRACTICE to determine both butanol-extractable iodine (BET) and cholesterol on a given sample of serum. Recently, Man proposed adding thiouracih in approximately 0.OO1M concentration to stored sera as a preservative for BET determinations (1). An impression was gained in the author’s laboratory that sera so treated with thiouracil often yielded cholesterol values that appeared to ...

Broiler chickens were reared in batteries and fed diets designed to determine the effects of either ascorbic acid, aspirin (acetylsalicylic acid), lysine, or thiouracil on thyroid weight and serum thyroid hormone concentrations. Thyroxine (T4) and 3,5,5'-triiodothyronine (T3) concentrations in serum were determined by radioimmunoassay. Neither ascorbic acid nor lysine affected T3 or T4 concentr...

By LOUIS R. LIMARZI, M.D., RICHARD J. KULASAVAGE, M.D., AND CONRAD L. PIRANI, M.D. O NE of the toxic reactions of therapeutic doses of thiouracilt in the treatment of hyperthyroidism is the development of granulocytopenia. A nonfatal granulocytopenia and a fatal type of agranulocytosis have been reported. The selective action of thiouracil on the granulopoietic tissue is the basis for the prese...

In a survey of various substrates for pyrimidine nucleoside synthesis, it was noted that inorganic orthophosphate was released when 2-thiouracil and desoxyribose-l-phosphate were incubated together with thymidine phosphorylase (1). This finding, taken as a fairly certain indication of nucleoside synthesis, has now been fully substantiated by isolation and characterization of crystalline thioura...

Previous work in this laboratory (1) has shown that an increase in carbamyl phosphate synthetase activity in the livers of thyroxine-treated tadpoles is the result of protein synthesis de 1u)vo. This induction of the synthesis of a specific enzyme protein by thyroxine was found to be inhibited by chloramphenicol and thiouracil (2). A subsequent survey (3) of several purine and pyrimidine deriva...

Transfer RNAs isolated from Escherichia coli B grown in the presence of 2-thiouracil are deficient in pseudouridine. Much of this deficiency is from the T psi C region, which has only about 50% of its normal pseudouridine content. The other modified nucleoside from this region, ribothymidine, is reduced by only about 10%. Studies showed that 2-thiouracil is incoproated into the RNA of E. coli d...

The electrochemical oxidation of methyl catechol in the presence of 2-thiouracil (as a nucleophile) has beenperformed in aqueous solutions using cyclic voltammetry and controlled-potential coulometric techniques.The electrochemical behavior of methyl catechol in the presence of 2-thiouracil was investigated. Theelectrochemical synthesis of this compound has been successfully performed in an und...

The applicability of Cohn's method number 10 to the study of dog plasma has made possible this investigation of experimental canine atherosclerosis. Administration of thiouracil which causes hypercholesterolemia but no atherosclerosis in dogs produces no considerable alteration in the distribution of plasma lipoproteins. High cholesterol feeding and administration of thiouracil causes both athe...