A novel murine NK cell-reactive mAb, AT8, was generated. AT8 recognizes Ly49G from 129/J, BALB/c, and related mouse strains, but does not bind to Ly49G(B6). Costaining with AT8 and a Ly49G(B6)-restricted Ab (Cwy-3) provides the first direct evidence that Ly49G protein is expressed from both alleles on a significant proportion of NK cells from four different types of F(1) hybrid mice. The observ...

NK cells obtained by exposing mouse fetal thymocytes to appropriate combinations of IL-4, IL-2, and PMA are phenotypically indistinguishable from cultured adult splenic NK cells with the exception that they generally lack measurable expression of all of the inhibitory Ly49 molecules that can currently be detected with Abs (Ly49A, -C, -G, and -I) and of the activating molecule Ly49D. Despite thi...

Chemical cell lysis is an interesting topic in the research to Lab-on-a-Disc (LOD) platforms on account of its perfect compatibility with the centrifugal spin column format. However, standard procedures followed in chemical cell lysis require sophisticated non-contact temperature control as well as the use of pressure resistant valves. These requirements pose a significant challenge thereby mak...

Whole blood was heated for twenty minutes at 40 degrees C., 45 degrees C., 50 degrees C., and 55 degrees C. Changes in the osmotic resistance of the heat-exposed cells were then determined. The values obtained were plotted as lysis increments. This treatment revealed groups of cells of varying heat sensitivity. After heating to 50 degrees C., cell groups were therefore prepared by partitioning ...

Thermal hydrolysis pre-treatment of sludge for anaerobic digestion increases biogas production, enhances volatile solids reduction, and improves dewaterability of digested sludge. This study reports the performance of a thermochemical hydrolysis process (TCHP) that utilizes heat (60°C70 °C) and sodium hydroxide for cell lysis. The objective of this study is to evaluate the impacts of implementi...

42 Background: This is a rare instance of acute kidney injury caused by hyperuricemia due to 43 spontaneous tumor lysis syndrome and also the first case of spontaneous tumor lysis syndrome 44 reported in association with myelodysplastic syndrome. Case Presentation: A 53-year-old man 45 presented with abrupt oliguria. Laboratory findings on admission included hyperuricemia, 46 hyperphosphatemia,...

Recombinant plasminogen activator (reteplase) is a third generation thrombolytic agent which has been used on coronary artery thrombosis and acute myocardial infarction. Clot lysis assay is usually considered as a unique method to evaluate biological activity of reteplase. In this study biological activity of reteplase was determined by APTT (activated partial thromboplastin time) lysis method....

Cell lysis of Gram-negative bacteria can be efficiently achieved by expression of the cloned lysis gene E of bacteriophage PhiX174. Gene E expression is tightly controlled by the rightward lambda pR promoter and the temperature-sensitive repressor cI857 on lysis plasmid pAW12. The resulting empty bacterial cell envelopes, called bacterial ghosts, are currently under investigation as candidate v...

The Ly-49 gene families are class I-recognizing receptors on murine NK cells. Most Ly-49 receptors inhibit NK cell lysis upon recognizing their target class I ligands. In this report we have examined the ability of Ly-49A and Ly-49G2 to regulate T cell functions on CD3+ cells, primarily the subset that also expresses NK-1.1 and/or DX5. The majority (>50%) of T cells that express Ly-49 molecules...