Crown gall tumors develop after integration of the T-DNA of virulent Agrobacterium tumefaciens strains into the plant genome. Expression of the T-DNA-encoded oncogenes triggers proliferation and differentiation of transformed plant cells. Crown gall development is known to be accompanied by global changes in transcription, metabolite levels, and physiological processes. High levels of abscisic ...

The habituation of soybean (Glycine max) callus can be induced rapidly, by exposing the tissue to small amounts (10(-9)molar) of compounds including 2.4-dinitrophenol and phenoxyisobutyric acid for brief periods of time. Such compounds reportedly exhibit antiauxin activity. Various soybean callus phenotypes have been isolated which continue to exhibit hormone habituation 14 months following the...

Renaturation kinetics of labeled Agrobacterium tumefaciens DNA are not influenced by addition of 10(4)-fold excess of crown gall tumor DNA. Reconstruction experiments demonstrated that 0.01% added bacterial DNA produces a detectable increase in rate of renaturation of labeled DNA. Crown gall tumor DNA therefore cannot contain as much as 0.01% A. tumefaciens DNA (one entire bacterial genome per ...

BACKGROUND Agrobacterium vitis is a causal agent of crown-gall disease. Trifolitoxin (TFX) is a peptide antibiotic active only against members of a specific group of alpha-proteobacteria that includes Agrobacterium and its close relatives. The ability of TFX production by an avirulent strain of Agrobacterium to reduce crown gall disease is examined here. RESULTS TFX was shown to be inhibitory...

The formation of tumors in tissues of higher plants by the crown-gall organism, (Phytomonas tumefaciens), is influenced by several factors. First, the bacteria themselves must be virulent. Avirulent strains of the crown-gall organism will not produce a gall in susceptible tissue unless that tissue is also stimulated by a growth hormone (1, 10) or by products from a gall produced by fully virule...

Green shoot cuttings of 10 different grapevine species were inoculated with Agrobacterium vitis to find disease-related metabolites in the grapevine. Crown galls formed 60 days after inoculation varied in gall severity (GS) evaluated by gall incidence (GI) and gall diameter (GD), which were classified into three response types as RR (low GI and small GD), SR (high GI and small GD), and SS (high...

Crown gall caused by Agrobacterium tumefaciens is the major limiting factor in raising healthy stone fruit plants in nurseries. The incidence of crown gall varies from 4 to 97.5 per cent at different locations resulting in out right rejection of an average of 30 per cent stone fruit plants in nurseries. Agrobacterium radiobacter strain K-84 has been reported to provide nearly complete control o...

Several kinds of primary sunflower (Helianthus annuus) crown gall tissues were established in tissue culture and then labeled in vivo with either [(14)C]arginine, [(14)C]histidine, [(3)H]lysine, or [(3)H]ornithine. Crown gall tissues incited by Agrobacterium tumefaciens strains that utilize octopine as a sole source of carbon or nitrogen for growth synthesized the four members of the N(2)-(1-ca...

cultivar of roses (rosa spp.) has been grown in greenhouses in qazvin region of iran for local markets. agrobacterium tumefaciens strains were isolated and identified from six different samples of roses plants imported from the netherland to iran. during august and september of 2012, nearly 2-5% of rose plants in two different greenhouses in the province of qazvin were observed with crown gall ...