BACKGROUND Adipose tissue damage of cryopreserved fat after autologous fat transfer is inevitable in several processes of re-transplantation. This study aims to compare and analyze the survivability of adipocytes after thawing fat cryopreserved at -20℃ by using thawing methods used in clinics. METHODS The survival rates of adipocytes in the following thawing groups were measured: natural thaw...

objective: vitrification has been shown as one of the most effective methods of cryopreservation for mammalian embryos. however, there is no consensus which stage of embryonic development is the most appropriate for vitrification with subsequent maximal development after thawing. this study was carried out to explore and compare the effect(s) of vitrification on mouse 2-cell, 4-cell, 8-cell, mo...

The objective was to determine the effect of sequence of insemination after simultaneous thawing of multiple 0.5 mL semen straws on conception rate in suckled multiparous Nelore cows. The effect of this thawing procedure on in vitro sperm characteristics was also evaluated. All cows (N = 944) received the same timed AI protocol. Ten straws (0.5 mL) of frozen semen from the same batch were simul...

We report on the preparation of both porous and non-porous poly(vinyl alcohol) (PVA) hydrogels that can be used as carriers to release proteins. Non-porous gels were prepared by freezing and thawing of aqueous PVA solutions, while porous gels were formed by adding porosigens such as NaCl or ammonium carbamate into aqueous PVA solutions. Bovine serum albumin, a model protein, was loaded into non...

objective: the aim of the present study was to evaluate three thawing rates on the post thaw motility, viability and chromatin structure of buffalo semen frozen in 0.5-ml straws. materials and methods: in this experimental study semen was collected with artificial vagina (42˚c) from four buffalo bulls.split pooled ejaculates (n=4) were extended at 37˚c with a bioxcell® extender. semen was coole...

Dalton et al. (2004) conducted a field trial with Holstein dairy cattle to determine: 1) the effect of simultaneous thawing of multiple 0.5-mL straws of semen and sequence of insemination (1, 2, 3 or 4) on conception rates, 2) whether conception rates achieved following AI by professional AI technicians (PAI) and herdsman-inseminators (HI) differed, and 3) the effect of elapsed time from initia...

The effects of different freezing and thawing rates on the post-thaw motility and membrane integrity of boar spermatozoa, processed as split samples in Maxi-straws or flat PET-plastic packages (FlatPack) were studied. A programmable freezing device was used to obtain freezing rates of either 20, 50 or 80 degrees C/min. Thawing of the samples was performed in a bath of circulating water; for 40s...

BACKGROUND The purposes of this study were to compare the survival rate of human blastocysts thawed by two different methods after vitrification using electron microscopic (EM) grids, and to report the successful pregnancies and live births resulting from the transfer of blastocysts that had survived the established thawing method. METHODS The blastocysts produced from three pronuclei zygotes...