In this paper, we compare ndh genes in the plastid genome of many Cymbidium species and three closely related taxa in Orchidaceae looking for evidence of ndh gene degradation. Among the 11 ndh genes, there were frequently large deletions in directly repeated or AT-rich regions. Variation in these degraded ndh genes occurs between individual plants, apparently at population levels in these Cymbi...

Sclerotium rot was found on Cymbidium orchids at Seosan-si, Chungcheongnam-do, Korea, in July, 2010. Symptoms occurred on low leaves, which turned yellowish, after which the entire plant wilted. Severely infected plants were blighted and eventually died. White mycelial mats and sclerotia appeared on pseudobulbs. Based on the mycological characteristics and pathogenicity, the causal fungus was i...

The FLOWERING LOCUS T (FT) gene plays crucial roles in regulating the transition from the vegetative to reproductive phase. To understand the molecular mechanism of reproduction, three homologous FT genes were isolated and characterized from Cymbidium sinense "Qi Jian Bai Mo", Cymbidium goeringii and Cymbidium ensifolium "Jin Si Ma Wei". The three genes contained 618-bp nucleotides with a 531-b...

The protocorm-like bodies (PLBs) of Cymbidium hookerianum were used as the explants, effects different hormones and hormone concentrations on induction etiolation stems, subsequent regeneration artificial seeds production conservation using stems studied. results showed that most efficient induced by PLBs was obtained adding 0.1 mg/L NAA 6-BA. cultivated medium containing 2.0/0.1 NAA/6-BA highe...

In this paper, we investigated the chemical components of the flowers of Cymbidium Lunagrad Eternal Green for the first time. In the whole post-fertilization, a new alkaloid, named Lunagrad A (1), and a new aromatic glucoside, named Lunagrad B (2), were isolated from the MeOH extract of the flowers of Cymbidium Lunagrad Eternal Green, along with other six known aromatic compounds (3-8) and thre...

Protocorm pieces of the orchid Cymbidium were aseptically cultured either without phytohormones, or with one of the growth promoting substances, auxin cytokinin, and gibberellin. The derivative melting profiles of the extracted DNA's differ from each other with respect to the size of various AT- and GC- rich fractions. Evidence has been obtained for the increase of the more AT--rich fractions i...

In 2006~2010, leaf spot symptoms, that is, small, yellow spots that turned into dark brown-to-black lesions surrounded by a yellow halo, were observed on Cymbidium spp. in Gongju, Taean, and Gapyeong in Korea. A Fusarium species was continuously isolated from symptomatic leaves; in pathogenicity testing, isolates caused leaf spot symptoms consisting of sunken, dark brown lesions similar to the ...

The population of many splendid orchids is reducing from their natural habitats at an alarming rate and their conservation is becoming a matter of global concern. Asymbiotic seed germination has been applied for ex situ conservation of rare, endangered and threatened orchid taxa and could provide rapid means their multiplication. In the present study reported here, seeds of an epiphytic and rar...

The demand for sustainable agricultural production systems is increasing, and using growth-promoting microorganisms in plants has stood out because it decreases or even replaces chemical fertilizer use, reducing costs. This study aimed to evaluate the response of some applied seedlings primary orchids cultivated Brazil (Phalaenopsis sp. 'Taisuco Swan', Cymbidium atropurpureum, Dendrobium secund...

A comparative study of in vitro seed germination of two endangered orchid species, viz. Cymbidium elegans Lindl. and Dendrobium densiflorum Lindl. ex Wall., was carried out on Murashige and Skoog’s (MS) medium, supplemented with different concentrations and combination of 6-benzylaminopurine (BAP) and á-Naphthalene acetic acid (NAA). The hormone-free MS medium and MS medium supplemented with va...