enhanced oxidative stress in diabetes mellitus may contribute to the pathogenesis of diaetic complications. in this article, the stress oxidative generation has been studied in experimental diabetes by ferric reducing ability of plasma assay, a sensitive and simple method, and by other oxidative damage markers. the ferric reducing ability of plasma values as a total antioxidant capacity were si...

BACKGROUND Currently available clinical tools cannot accurately identify the extent of perinatal hypoxic injuries. During hypoxia, reactive oxygen species cause lipid peroxidation of cell membranes, yielding oxidation products that constitute thiobarbituric acid-reacting substances (TBARS). OBJECTIVE To see if the concentrations of TBARS excreted in urine would be elevated during the first da...

Introdução: O tabagismo é considerado uma epidemia mundial e, segundo a Organização Mundial de Saúde (OMS), cerca cinco milhões pessoas morre cada ano em consequência das doenças relacionadas ao tabaco. tabaco, além vários efeitos nocivos, está relacionado com aumento células defesa. Objetivo: Avaliar viabilidade polimorfonucleares (PMNs) e o dano oxidativo no sangue indivíduos fumantes não atr...

OBJECTIVES This study investigated serum thiobarbituric acid-reactive substances (TBARS) and free thiol levels in different subtypes of acute ischemic stroke (AIS) and evaluated their association with clinical outcomes. METHODS This prospective study evaluated 100 AIS patients, including 75 with small-vessel and 25 with large-vessel diseases. Serum oxidative stress (TBARS) and antioxidant (th...

OBJECTIVE We determined the calcium activated adenosine triphosphatase (Ca-ATPase) activity and level of lipid peroxidation thiobarbituric acid-reactive substances (TBARS) of red blood cell ghosts in the antepartum and postpartum of normotensive and preeclamptic pregnant women. METHODS Samples of venous blood were obtained by venipuncture of nulliparous normotensive and preeclamptic pregnant ...

Malondialdehyde (MDA) is one of the most commonly reported biomarkers of lipid peroxidation in clinical studies. The reaction of thiobarbituric acid (TBA) with MDA to yield a pink chromogen attributable to an MDA-TBA₂ adduct is a common assay approach with products being quantified by ultraviolet-Vis assay as nonspecific TBA-reactive substances (TBARS) or chromatographically as MDA. The specifi...

BACKGROUND/AIMS Nephropathic cystinosis (NC) is a severe systemic disease and cysteamine improves its prognosis. Lysosomal cystine accumulation is the hallmark of cystinosis and is regarded as the primary defect due to mutations in the CTNS gene. However, there is great evidence that cystine accumulation itself is not responsible for all abnormalities observed in NC. Studies have demonstrated a...

A simple, reliable, and reproducible fluorometric method for measuring thiobarbituric acid-reactive substances (TBARS) in serum is proposed, based on the reaction between malondialdehyde (MDA) and thiobarbituric acid. Formation of TBARS was complete at pH 2.4-2.6, but extraction with n-butanol proved complete only at lower pH, i.e., 1.6-1.7. Analytical recoveries of MDA added to serum were 94%-...

OBJECTIVE To investigate the lipid peroxidation (PEROX) processes in primary open-angle glaucoma (POAG) patients, and whether this mechanism may be related to disease progression. MATERIAL AND METHODS A prospective, observational, cross-sectional, non-experimental, and analytical study was conducted on a case and a comparison group, consisting of 175 surgical patients divided into: 1) POAG gr...