Tailing of DNA with terminal transferase is a technically demanding, but essential step in full-length cDNA cloning procedures*•^. It is also an essential step of a poly(dA) affinity labelling technique, which has been used to select families of partial restriction fragments on oligo(dT)cellulose^•*. In order to bypass the need for transferase in the latter technique, we made a simple modificat...

Rare earth mine tailing dumps are environmental hazards because tailing easily leaches and erodes by water and wind. To assess the influence of mine tailing on the geochemical behavior of rare earth elements (REEs) in soil, sixty-seven surface soil samples and three soil profile samples were collected from different locations near China's largest rare earth mine tailing. The total concentration...

Non-specific tail sequences are often added to the 5'-terminus of primers to improve the robustness and overall performance of diagnostic assays. Despite the widespread use of tailed primers, the underlying working mechanism is not well understood. To address this problem, we conducted a detailed in vitro and in silico analysis of the enhancing effect of primer tailing on 2 well-established foo...

RFID (Radio-Frequency IDentification) is a key emerging technology for supply-chain monitoring and detection of counterfeit and grey-market goods. The most prevalent RFID tags are, however, simply “wireless barcodes,” themselves vulnerable to cloning and counterfeiting. While continuous monitoring can, in principle, detect cloning attacks, real-world supply chains often contain significant blin...

Workability is a key performance criterion for mining cemented tailing backfill, which should be defined in terms of rheological parameters such as yield stress and plastic viscosity. Cemented tailing backfill is basically composed of mill tailings, Portland cement, or blended cement with supplementary cement material (fly ash and blast furnace slag) and water, among others, and it is important...

Changes in the tailing properties (increasing clay mineral content and fine particles) and poor operation of the dewatering systems have negative impacts on the Tailing Storage Facilities (TSF) of the Shahrbabak copper complex. The design solid concentration of the thickened tailings is 63 wt.% in the Shahrbabak paste plant but it is well below the design value right now (approx. 55 wt.%). The ...

The inevitable depletion of mineral resources, the constant deterioration of the geological and mining conditions for the development of mineral deposits and the restoration of raw materials from mining waste by recycling are all urgent problems we face today. The solution to this problem may ensure: a considerable extension of raw material source; decrease of investments in opening new deposit...

This paper may be of particular interest to the readers as it provides a new environmental risk assessment system for phosphogypsum tailing dams. In this paper, we studied the phosphogypsum tailing dams which include characteristics of the pollution source, environmental risk characteristics and evaluation requirements to identify the applicable environmental risk assessment methods. Two analyt...

E-tailing websites are often perceived to be static, impersonal and distant. However, with the movement of the World Wide Web to Web 2.0 in recent years, these online websites have been found to display personalities akin to ‘humanistic’ qualities and project impressions much like its retailing counterpart i.e. salespeople. This paper examines the personality of e-tailing websites and their imp...

First, concentrating on the seasonal (s = 52) lags, the ACF shows a strong peak at h = 1s and lesser, but still statistically significant peaks at h = 3s , 4s, 5s, and 7s. The PACF shows statistically significant peaks at only h = 1s. It appears that: • the ACF is cutting off after lag 1s and the PACF is tailing off in the seasonal lags • the ACF is cutting off after lags 3s, 4s, 5s, or 7s, and...