Sequence tagged site (STS) markers have been developed recently to identify resistance genes in wheat. A number of wild relatives have been used to transfer resistance genes into wheat cultivars. Accessions of wild species of Triticeae: Aegilops longissima (4), Ae. speltoides (6), Ae. tauschii (8), Ae. umbellulata (3), Ae. ventricosa (3), Triticum spelta (2), T. timopheevi (3), T. boeoticum (4)...

The aldehyde tag is appropriate to selectively label proteins, prepare antibody-drug conjugates or immobilize enzymes antibodies for biotechnological and medical applications. cysteine within the consensus sequence CxPxR of specifically oxidized by formylglycine-generating enzyme (FGE) non-canonical electrophilic amino acid Cα-formylglycine (FGly). Subsequent reductive amination a common method...

Three unusual mortalities events involving bottlenose dolphins (Tursiops truncatus Montagu 1821) occurred along Florida’s northern Gulf of Mexico coast between 1999 and 2006. The causes these events, in which over 300 are known to have died, still under investigation. impact mortality cannot be fully evaluated, because little prior information on dolphin abundance distribution patterns exist th...

Abstract The European bass (Dicentrarchus labrax) support high value commercial and recreational fisheries, however the Spawning Stock Biomass (SSB) of northern Atlantic stock (ICES divisions 4.b–c, 7.a, 7.d–h) has rapidly declined to an unsustainable level. decline in SSB been attributed fishing pressure combined with poor recruitment. By tracking juvenile fish their spatial ecology can be ide...

First posted October 28, 2021 For additional information, contact: Director, Western Fisheries Research CenterU.S. Geological Survey6505 NE 65th StreetSeattle, Washington 98115-5016 A telemetry study was conducted during August–December 2020 to evaluate behavior and movement patterns of adult smallmouth bass (Micropterus dolomieu) in the forebay Bonneville Dam, Washington. total 40 were collect...

The paper describes work on verifying, correcting and retagging a corpus of Amharic news texts. A total of 8715 Amharic news articles had previously been collected from a web site, and part of the corpus (1065 articles; 210,000 words) then morphologically analysed and manually part-of-speech tagged. The tagged corpus has been used as the basis for testing the application to Amharic of machine l...

The site-specific immobilization of histidine-tagged proteins to patterns formed by far-field and near-field exposure of films of aminosilanes with protein-resistant photolabile protecting groups is demonstrated. After deprotection of the aminosilane, either through a mask or using a scanning near-field optical microscope, the amine terminal groups are derivatized first with glutaraldehyde and ...

Production of milligram quantities of numerous proteins for structural and functional studies requires an efficient purification pipeline. We found that the dual tag, his(6)-tag-maltose-binding protein (MBP), intended to facilitate purification and enhance proteins' solubility, disrupted such a pipeline, requiring additional screening and purification steps. Not all proteins rendered soluble by...