An enzyme electrode was developed using the enzyme sulfite oxidase (EC1.8.3.1) immobilized onto pig intestine. Hydrogen peroxide produced was monitored amperometrically. All experimental parameters such as pH, temperature, buffer constituent concentration, were thoroughly investigated and optimized when appropriate. The response to sulfite concentration was linear in the range 5.2´...

A method for measuring sulfite residues in foods by using the modified Rankine apparatus and HPLC was developed. Sulfites released from foods by bubbling of nitrogen gas and heating for 15 minutes with an electric burner weRe absorbed in 1% triethanolamine solution and detected as sulfite ion by HPLC with a UV detector at 240 nm, using an anion exchange column, IonPac AS9-SC, with 1.8 mmol/L so...

By coupling the bromate-sulfite-ferrocyanide oscillating chemical reaction with the complexation of calcium ion by EDTA, we construct a system that generates periodic pulses of free Ca(2+) with an amplitude of 2 orders of magnitude and a period of ca. 20 min. These pulses may be observed either with a calcium ion-selective electrode or with Arsenazo(III) as an indicator. We describe the systema...

A sulfur:ferric ion oxidoreductase that utilizes ferric ion (Fe3+) as an electron acceptor of elemental sulfur was purified from iron-grown Thiobacillus ferrooxidans to an electrophoretically homogeneous state. Under anaerobic conditions in the presence of Fe3+, the enzyme reduced 4 mol of Fe3+ with 1 mol of elemental sulfur to give 4 mol of Fe2+ and 1 mol of sulfite, indicating that it corresp...

Energy metabolism of the alkaliphilic sulfate-reducing bacterium Desulfonatronovibrio hydrogenovorans strain Z-7935 was investigated in continuous culture and in physiological experiments on washed cells. When grown in chemostats with H2 as electron donor, the cells had extrapolated growth yields [Y(max), g dry cell mass (mol electron acceptor)(-1)] of 5.5 with sulfate and 12.8 with thiosulfate...

in this research, an enzyme modified electrode has been produced during the electropolymerization of aniline through incorporation of sulfite oxidase into a conducting polymer. then the bioelectrochemical response of resulted sulfite biosensor was investigated at different experimental conditions. study of  the stability of the resulted sulfite biosensor revealed that formation of a passive fil...