Previous work from this laboratory has indicated that thrombin's influence on cell growth can be negative as well as positive. Addition of enzyme to actively growing or confluent cultures of human skin fibroblasts produced growth stimulation, whereas cultures receiving thrombin at the time of subculture displayed inhibited DNA synthesis and mitosis. The specific binding of [125I]thrombin to cel...

Cell differentiation degree and mitotic activity were sequentially assessed by immunoperoxidase labeling of glial fibrillary acidic protein (GFAP) and proliferative cell nuclear antigen (PCNA), respectively, in rat brain cultured astrocytes maintained up to 60 days in vitro (DIV) of first subculture, or weekly passaged until their 12th subculture. Cell count was performed through a 0.01 mm2 sec...

An efficient and reproducible procedure was established for direct shoot regeneration of Solanum surattense Bum. using internodal explants. The shoot regeneration efficiency on Murashige and Skoog (MS) medium supplemented with 6benzylaminopurine (BAP) was superior in compared to 6furfurylaminopurine (Kn). The highest mean number of shoots per explant was recorded on MS medium containing 0.5 mgl...

OBJECTIVE To investigate whether deliberate self harm is associated with contemporary Goth youth subculture. DESIGN Longitudinal cohort study. SETTING School and community based study of young people living in the Central Clydeside Conurbation, Scotland. PARTICIPANTS 1258 people aged 19, surveyed in 2002-4 and followed-up since age 11 (1994). MAIN OUTCOME MEASURES Lifetime prevalence of...

A study of 16,108 blood cultures investigated the value of performing early smears in addition to the inspection of early subcultures after 12 h of incubation. The early smear and subculture detected 44.6% of 1,236 positive cultures; of those detected by the early subculture, 57.3% grew after 12 h of incubation.

The maintenance of Leptospira species in liquid or semisolid medium is time-consuming and at risk of contamination due to the needs of routine subculture and dark field microscopy. Using Leptospira Vanaporn Wuthiekanun (LVW) agar, we maintained 100 pathogenic Leptospira isolates for 12 months without the need for subculture and confirmed the viability of all isolates by the naked eye.

Youth subcultures, characterized by a distinctive lifestyle, music preference, shared values and behaviors, are associated with substance use. The aim of this study was to explore whether protective factors such as parental monitoring, parental bonding and parental substance abstinence affect the association between subculture affiliation and adolescents' substance use. We used data from 15-yea...