We used a polyclonal antiserum directed against calpain II to study the distribution of that enzyme in rat sciatic nerve. Western blot of nerve homogenate showed that the antibody reacted with a single protein band of 80 kDa, corresponding to the catalytic subunit of calpain II. By light microscopy, immunoreactivity appeared predominantly in Schwann cell cytoplasm. By electron microscopy, calpa...

introduction: the aim of this study was to investigate the scanning electron microscope (sem) analysis of tooth surface irradiated by different parameters of er:yag laser. methods: 15 caries-free extracted human third molars were used in this study. the teeth were put into 5 groups for laser irradiation as follows: group 1 (power: 2.5 w, energy: 250 mj); group 2(power: 3 w, energy: 300 mj); gro...

purpose: to determine the dissection level during deep lamellar keratoplasty (dalk) using the big-bubble technique by light microscopy (lm) and transmission electron microscopy (tem). methods: descemet’s membrane (dm) and stromal tissue of 4 keratoconic eyes were removed because of inadvertent intraoperative dm perforation during dalk which was converted to penetrating keratoplasty. these speci...

Five different enzyme immunoassays, electron microscopy, and latex agglutination (Slidex; bioMerieux) were compared for the rapid detection of human rotavirus in fecal specimens. The enzyme immunoassay using rotavirus polyclonal antiserum (Dakopatts) with simple in-house modifications was shown by the use of confirmatory tests to be the most sensitive and specific procedure.

A monospecific antiserum to tobacco plastocyanin agglutinates stroma-free swellable chloroplasts from wild type tobacco, (Nicotiana tobacum var. John William's Broadleaf) from the tobacco aurea mutant Su/su2, (Nicotiana tabacum var. Su/su2) from Antirrhinum majus and spinach (Spinacia oleracea). In this condition the antiserum inhibits linear photosynthetic electron flow in tobacco and spinach ...

In this paper many of the principal struc­ture of the Moral Lung were studied with the help of Electron Microscopy. Detailed descrip­tion was given of Granular Pneumocytes, Membranous Pneumocytes, Macrophages, and Alveolar Capillaries. A discussion followed on the form and function of Granular Pneumo­cytes.  Acknowledgements  I am grateful to the Reza Shah Pahlavi Foundation for its generou...

objective: confocal laser scanning microscopy (clsm) is relatively a new light microscopical imaging technique with a wide range of applications in biological sciences. primary value of clsm for the biologist is its ability to provide optical sections from three-dimensional specimen. the present study was designed to assess the thickness content of in vivo accumulated dental plaque using clsm a...

background:neonatal calf diarrhea (ncd), also known ascalf scours, is caused by viruses, bacteria, and parasites.coronavirus is one of the important causes of ncd syndrome.electron microcopy has been used for demonstration of virusesin fecal samples of diarrheic calves since 1969. objectives:the aim of this study was to determine the prevalence ofcoronavirus in fecal samples of neonatal calf di...