Adeno-associated virus vectors (AAV) are reported to have a great potential for gene therapy, however, major bottleneck this kind of therapy is the limitation production capacity. Higher specific AAV vector yield often adherent cell systems compared cells in suspension, and microcarrier-based culture well established anchored on larger scale. The purpose present study was explore how microcarri...

The effect of organic and inorganic nitrogen sources on Trichoderma reesei Rut-C30 cellulase production was investigated in submerged cultivations. Stirred tank bioreactors and shake flasks, with and without pH control, respectively, were employed. The experimental design involved the addition of individual organic nitrogen sources (soy peptone, glutamate, glycine and alanine) within a basal me...

This thesis focuses on the design and characterisation of miniature bioreactors and evaluates their potential as a scale-down device for microbial cultivation processes. Miniature bioreactors, such as the one detailed in this work, have been developed by many research groups and companies, and seek to increase throughput at the early stages of bioprocess development. Power input was measured in...

We have developed an integrated array of microbioreactors, with 100 microL working volume, comprising a peristaltic oxygenating mixer and microfluidic injectors. These integrated devices were fabricated in a single chip and can provide a high oxygen transfer rate (k(L)a approximately 0.1 s(-1)) without introducing bubbles, and closed loop control over dissolved oxygen and pH (+/-0.1). The syste...

A stirred tank bioreactor (STB) integrated with an expanded bed adsorption (EBA) system containing anion-exchange resin (Diaion WA30) was developed for in situ removal of acetate to increase the production of α-interferon-2b (α-PrIFN-2b) by Escherichia coli (E. coli). Although the total acetate (9.79 g/L) secreted by E. coli in the integrated STB/EBA system was higher than that in a bioreactor ...

The modified rotating simplex method has been successfully used to determine the best combination of agitation rate and aeration rate for maximum production of extracellular proteases by Staphylococcus aureus mutant RC128, in a stirred tank bioreactor operated in a discontinuous way. This mutant has shown altered exoprotein production, specially enhanced protease production. Maximum production ...

2015 Aim: Bioreactors are an essential component in every biotechnological process. Due to the multitude of different microbial and mammalian organisms used for production of complex products, novel concepts for customized cultivations are necessary. Results: A ceramic capillary-based bioreactor enabling a novel approach for steadystate biofilm cultivation is presented. A model for the determin...

Virtual laboratories have successfully proven to be very versatile and intuitive when simulating experiments in science, biotechnology, engineering. These tools must complement the carried out real labs or pilot plants. This study describes creation of a virtual laboratory through Easy JavaScript Simulation platform. A web-based simulation an enzymatic stirred-tank bioreactor has been built usi...

The microbial degradation of thiodiglycol, the primary hydrolysis product of sulfur mustard, by a pure culture of Alcaligenes xylosoxydans ssp. xylosoxydans (SH91) was accomplished in laboratory scale stirred tank reactors. This is a major component of the overall biodegradation process proposed for the complete mineralization of sulfur mustard. Several configurations were evaluated for degrada...

Human mesenchymal stem cells (hMSCs) are manufactured as cell therapy products. This requires a tightly controlled and fully understood production process, usually taking place in stirred-tank bioreactor. In this review, the impact of bioreactor geometry well equipment-related parameters is discussed. We provide theoretical background for engineering hMSC spheroids with defined size evaluate pr...