Rationale and significance. Recombinant therapeutic proteins are frequently produced in mammalian cells cultivated in suspension in large-scale stirred tank bioreactors. There are three major drawbacks to stirred-tank bioreactors. First, they are expensive to purchase and maintain, limiting their availability to some potential users. Second, they have not been adapted to volumetric scales less ...

Optimization of submerged culture conditions for the production of mycelial growth and exopolysaccharides (EPSs) by Collybia maculata was investigated. The optimum temperature and the initial pH for EPS production in a shake-flask culture of C. maculata were found to be 20°C and 5.5, respectively. Among the various medium’s constituents examined, glucose, Martone A-1, K2HPO4, and CaCl2 were the...

The effect of organic and inorganic nitrogen sources on Trichoderma reesei Rut-C30 cellulase production was investigated in submerged cultivations. Stirred tank bioreactors and shake flasks, with and without pH control, respectively, were employed. The experimental design involved the addition of individual organic nitrogen sources (soy peptone, glutamate, glycine and alanine) within a basal me...

A novel milliliter-scale stirred tank bioreactor was developed for the cultivation of mycelium forming microorganisms on a 10 milliliter-scale. A newly designed one-sided paddle impeller is driven magnetically and rotates freely on an axis in an unbaffled reaction vessel made of polystyrene. A rotating lamella is formed which spreads out along the reactor wall. Thus an enhanced surface-to-volum...

The autocatalytic reaction between chlorite and iodide ions exhibits a remarkable range of dynamical behavior. In a stirred tank reactor it shows bistability between steady states and between a steady and an oscillatory state. It forms the core of a large family of systematically designed chemical oscillators. The chlorite-iodide system has served as the prototype reaction in the discovery and ...

The turbulent flow field generated in a baffled stirred tank was computed by large eddy simulation (LED) and the flow field was developed using the Sliding Mesh (SM) approach. In this CFD study, mixing times and power number have been determined for a vessel agitated by a 6-blade Rushton turbine. The predicted results were compared with the published experimental data. The satisfactory results ...

This review describes the historical development and current state of metals leaching and sulfide mineral biooxidation by the minerals industries. During the past 20 years commercial processes employing microorganisms for mineral recovery have progressed from rather uncontrolled copper dump leaching to mineral oxidation and leaching in designed bioheaps for oxidation of refractory gold ores and...

Smoluchowski 's coagulation equation with coagulation rates K 0 oc (ij) ~ describing the temporal evaluation of the size distribution ck (concentration of k-mers) is studied in the presence of a source term ark, and a sink term --bCk. With a' a/b 2 one finds that a steady state is reached, exhibiting gelation when w > 1⁄2 and a ' > ao(w), where a0(o~) depends on w (ao(w) = ~ for ~o < 1⁄2, ao(1)...

Impeller stirred tanks (STR) are commonly used in the chemical processing industries (CPI) for a variety of mixing and blending technologies. In the present research, a numerical study of flow and mixing inside turbulently agitated tanks were carried out. An efficient solution algorithm was developed to solve turbulent flow inside stirred tanks where the boundary conditions for moving impeller ...