The question whether aminoacyl-tRNA synthetases act in a stepwise or in a concerted mechanism has been investigated kinetically with the valine enzyme of Escherichia coli, which had been used in previous studies by others who concluded that the physiological mechanism is concerted. An exchange between aminoacyl-tRNA and tRNA, dependent upon AMP, was studied. PPi inhibits this exchange completel...

The question whether aminoacyl-tRNA synthetases act in a stepwise or in a concerted mechanism has been investigated kinetically with the valine enzyme of Escherichia coli, which had been used in previous studies by others who concluded that the physiological mechanism is concerted. An exchange between aminoacyl-tRNA and tRNA, dependent upon AMP, was studied. PP-i inhibits this exchange complete...

Active self-assembly, in which non-thermal energy is consumed by the system to put together building blocks, allows the creation of non-equilibrium structures and active materials. Microtubule spools assembled in gliding assays are one example of such non-equilibrium structures, capable of storing bending energies on the order of 10(5) kT. Although these structures arise spontaneously in experi...

We offer a new perspective on games of irreversible investment under uncertainty in continuous time. The basis is a particular approach to solve the involved stochastic optimal control problems which allows to establish existence and uniqueness of an oligopolistic open loop equilibrium in a very general framework without reliance on any Markovian property. It simultaneously induces quite natura...

Abstract Biomembranes experience out-of-equilibrium conditions in living cells. Their undulation spectra are different from those thermal equilibrium. Here, we report on the of a fluid membrane pushed by stepwise growth filaments as leading edge migrating cells, using three-dimensional Monte Carlo simulations. The undulations largely modified equilibrium behavior. When tension is constrained, l...

Many economic environments are modeled as games under incomplete information. A fundamental concept in these models is the hypothesis of a Bayesian Nash equilibrium. However, in spite of the common use of this hypothesis in economic theory and econometric estimation models, no statistical methods have been suggested how to use real life data to test its validity. There are many doubts whether e...

The occurrence of significant second-order interactions for group characteristics was examined using real data in a randomized controlled trial (RCT). The interactions exist in all RCTs; they could be easily overlooked when using the simple randomization or stratification methods, but could become more obvious when minimization methods are used. Using real data from an RCT, the minimization met...