نتایج جستجو برای: step pcr using specific primers
تعداد نتایج: 4365160 فیلتر نتایج به سال:
Background: The aim of this study was compared the efficacy of the designed primers and already published primers for detection of the exoA, oprL and algD genes by PCR assay for finding a rapid, accurate and highly sensitive and specific procedure to detect the Pseudomonas aeruginosa in the serious and fatal infections such as cystic fibrosis disease, burned individual.Methods: A total of 150...
Invasive aspergillosis (1 is a life-threatening condition in immunocompromised patients. An early diagnosis is of great importance because early treatment may resolve this potentially fatal infection. Recently, the polymerase chain reaction (PCR) has been used successfully in detecting specific DNA of several pathogen. In this study, nested PCR was used to detect DNA specific for A!.pergiflus s...
Background: Meat species adulteration has become a problem of concern. This study aimed to compare two previously published multiplex Polymerase Chain Reaction (PCR) methods for meat species authentication. Methods: The primers used in the first multiplex PCR involved species-specific reverse primer for sheep, goat, cattle, pig, and donkey with universal forward primer. In the second multiple...
invasive aspergillosis (1 is a life-threatening condition in immunocompromised patients. an early diagnosis is of great importance because early treatment may resolve this potentially fatal infection. recently, the polymerase chain reaction (pcr) has been used successfully in detecting specific dna of several pathogen. in this study, nested pcr was used to detect dna specific for a!.pergiflus s...
during 1387-86, total number of 120 blood samples gathered from sheep with bluetongue-like clinical sign. the samples collected from seropositive regions .after separating serum, they were evaluated by competitive elisa for detecting ab against vp7 antigen. the full length of s7 gene (1156 bp) amplified by one step rt-pcr .in this method two sets specific primers, targeting 3? and 5?ends of s7 ...
Introduction: ESBL is an important factor in antibiotic resistance. Antibiotic resistance has been proposed as a global problem in recent decades and it causes many deaths all over the world; therefore, rapid and accurate diagnosis can be major step in solving this problem. This study aimed to identify SHV-type gene with PCR method. Methods and Materials: SHV gene sequ...
background: splicing by overlap extension (soe) pcr is used to create mutation in the coding sequence of an enzyme in order to study the role of specific residues in protein’s structure and function.objectives: we introduced a nested-soe-pcr (n –soe-pcr) in order to increase the specificity and generating mutations in a gene by soe-pcr. materials and methods: genomic dna from bacillus thermoca...
ABSTRACT- Desert truffles are hypogeous ascomyceteous ectomycorrhizal fungi, occurring in arid and semi-arid ecosystems. A PCR-based method was developed for the identification of 3 major desert truffles of Iran: Terfezia claveryi, Tirmania pinoyi and Tirmania nivea based on internal transcribed spacers of rDNA. Two specific PCR primers were designed for T. claveryi, 4 for T. pinoyi, and 2 for ...
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