Several esters of 4-methylumbelliferone and 2-naphthol were synthesized and examined as possible spectrofluorimetric titrants for bovine alpha-chymotrypsin, trypsin, thrombin, Factor Xa and for subtilisin Novo. 4-Methylumbelliferyl p-guanidinobenzoate hydrochloride (MUGB) is a satisfactory titrant for alpha- and beta-trypsin, thrombin and Factor Xa and 4-methylumbelliferyl p-(NNN-trimethylammon...

Introduction Chelate compounds, arising out of the combination of inorganic ions with polyfunctional organic molecules, may be expected to hold great interest for investigators in widely different fields of chemistry. The stability of many such substances can be an important factor both in the manipulation of ionic equilibria and in the alteration of organic structures. In the former applicatio...

As a part of our broad program of introducing new redox titrants in general and aromatic sulfonyl haloamines in particular, to develop rapid and accurate analytical procedures for determining a variety of industrially and biologically important organic and inorganic substances in solution (I, 3-25, 28, 30) we have introduced a new reagent, N,N-dibromobenzenesulfonamide or dibromamine-B (C,H,SO,...

The data indicate that trypsin-Sepharose has markedly different inhibition kinetics from trypsin in free solution. The Sepharose granules (i) offer protection from inhibition in the first place (e.g. for active-site titrants; Fig. Id) and/or (ii) facilitate the displacement of inhibitor from trypsin by excess substrate (Figs. la, Ib and Ic). It would seem that when an inhibitor such as ovomucoi...

Reports from various laboratories have dealt with the binding properties of glutamate dehydrogenase from bovine liver (EC 1.4.1.3) (Pantaloni & Dessen, 1969; Huang & Frieden, 1969; Malcolm, 1972; Brown et al., 1973; Koberstein & Sund, 1973), but unfortunately these reports differ considerably over the number and nature of glutamate dehydrogenase coenzyme-binding sites as well as the magnitude o...