نتایج جستجو برای: splicing by overlap extension pcr
تعداد نتایج: 7193854 فیلتر نتایج به سال:
epitope tagging is a method of expressing proteins whereby an epitope for a specific monoclonal antibody is fused to a target protein using recombinant dna techniques. the aim of this study was to sub-clone the peroxisomal protein (pep) cdna into a mammalian expression vector leading to the formation of a chimeric pep-cdna containing the flag epitope. the flag-pep recombinant cdna was construc...
This paper proposes and investigates overlap catenation as a formal model of a special case of cross-pairing PCR, an experimental procedure used to recombine two DNA strands by using the DNA Polymerase enzyme. This study is part of ongoing efforts to provide a formal framework and rigorous treatment of DNA-based information and DNA-based computation. Other studies along these lines include theo...
BACKGROUND Splicing by overlap extension (SOE) PCR is used to create mutation in the coding sequence of an enzyme in order to study the role of specific residues in protein's structure and function. OBJECTIVES We introduced a nested-SOE-PCR (N -SOE-PCR) in order to increase the specificity and generating mutations in a gene by SOE-PCR. MATERIALS AND METHODS Genomic DNA from Bacillus thermoc...
Brucellosis is a zoonotic disease in human and animals. Brucella melitensis is one of the most pathogenic species of Brucella in goat and sheep. Omp31 is an outer membrane protein of Brucella that acts as an immunogenic protein. Cytokines are glycoproteins with low molecular weight that play the role of an immune adjuvant and regulate immune responses. Interleukin-2 is one of the most important...
Objective(s): Tuberculosis (TB) is still one of the problematic infectious diseases in developing countries, especially in Iran. In the present study, we applied ribosome display technique to select single chain variable fragments (scFvs) specific for the 6-kDa early secretory antigenic target (ESAT-6) antigen of Mycobacterium tuberculosis from a mouse scFv library. Materials and Methods: The g...
NSLTP (nonspecific lipid transfer protein) ها پروتئین هایی گیاهی هستند که بر اساس وزن مولکولی به دو گروه nsLTP1 و nsLTP2 تقسیم بندی می شوند. این پروتئین ها بسیار مقاوم هستند و علاوه بر پتانسیل حمل داروها می توانند آن ها را در برابر اکسیداسیون یا تجزیه شدن محافظت کنند. مولکول nsLTP2 گیاه برنج ، پروتئینی است که به دلیل توانایی ذاتی عبور از غشا و اتصال به ترکیبات استروئیدی (از جمله برخی ترکیبا...
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