Using TSG101 pre-mRNA, we previously discovered cancer-specific re-splicing of mature mRNA that generates aberrant transcripts/proteins. The fact is aberrantly re-spliced in various cancer cells implies there must be an important mechanism to prevent deleterious on the spliced normal cells. We thus postulated controlled by specific repressors, and searched for repressor candidates siRNA-based s...

hypohidrotic ectodermal dysplasia (hed) is a rare congenital disorder arising from deficient development of ectoderm-derived structures including skin, nails, glands and teeth. the phenotype of hed is associated with mutation in eda, edar, edaradd and nemo genes, all of them disruptingnf-κb signaling cascade necessary for initiation, formation and differentiation in the embryo and adult. here w...

RNA splicing is the process of forming mature mRNA, which an essential phase necessary for gene expression and controls many aspects cell proliferation, survival, differentiation. Abnormal gene-splicing events are closely related to development tumors, generation oncogenic isoform in can promote tumor progression. As a main tumor-specific variants, alternative (AS) progression by increasing pro...

Abstract The study has developed a model splicing construct assay system based on misregulation, one of the major molecular features associated with myotonic dystrophy. double reporters for intron 2 in chloride channel (CLCN1). CLCN1 transgene was used to transfect wild type and DM fibroblast cell lines clones generated showed that it enabled quantification efficiency construct. Validation fibr...

Splicing systems are generative mechanisms introduced by Tom Head in 1987 to model the biological process of DNA recombination. The computational engine a splicing system is “splicing operation”, cut-and-paste binary string operation defined set rules”, quadruples [Formula: see text] where words over an alphabet text]. For two strings and text], applying rule produces In this paper we focus on ...

Abstract Splicing systems are a form of DNA computing as they mimic the recombination process among molecules. This work discusses use splicing to build automatic tools for reproducing human beings’ creativity , in context music composition . More specifically, this describes three general system approaches composition, and their application two specific cases, namely composing 4-voice Jazz sol...

Science in general, and specifically the science of life, offers an intriguing path to walk along. As you deepen your understanding a particular topic, become aware things did not see at all when started. Sometimes, this new seeing even drives reconsider redefine very basic concepts that learned along way. This is exactly what happened after we discovered process called RNA splicing. In splicin...

The transcription of the HIV-1 provirus results in only one type transcript—full length genomic RNA. To make mRNA transcripts for accessory proteins Tat and Rev, RNA must completely splice. Vif, Vpr, Env undergo splicing but not completely. Genomic (which also functions as Gag Gag/Pro/Pol precursor polyproteins) splice at all. can tolerate a surprising range relative abundance individual transc...

Introduction: Pathogenic variants of RUNX2, a gene that encodes an osteoblast-specific transcription factor, have been shown as the cause of CCD, which is a rare hereditary skeletal and dental disorder with dominant mode of inheritance and a broad range of clinical variability. Due to the relative lack of clinical complications resulting in CCD, the medical diagnosis of this disorder is challen...

Background: Pathological conditions, including ischemic stroke, are associated with severe Endoplasmic Reticulum (ER) stress that induces apoptosis and cell death. Herbal medicines are natural treatments with few side effects in such situations. Objectives: In the present study, we examined the probable neuroprotective effects of Viola spathulata, Lamium album, and Urtica dioica on splicing of...