Recent studies have shown that L-proline is a natural osmoprotectant and an antioxidant to protect cells from injuries such as that caused by freezing and thawing in many species including plant, ram sperm and human endothelial cells. Nevertheless, this nontoxic cryoprotectant has not yet been applied to mammalian oocyte vitrification. In this study we evaluated the efficiency and safety of the...

Background There are several indications of human female gamete cryostorage including sub-fertile and fertile patients. But our focus will be in women at risk of losing their reproductive function caused by oncologycal treatments or premature ovarian failure that could benefit greatly from this practice. Fertile women may take advantage of this technology to electively delay childbearing. The o...

Cryopreservation has the capacity to extend spermatozoa's lifespan and viability. In addition, the semen samples can be collected, preserved and stored or sent to distant locations and still be used long after the death of the semen donor. In this study for the vitrification of dog sperm (fresh and swum-up sperm), different cryopreservation mediums on the basis of glycerol, milk and egg yolk we...

The aim of this study was to develop and to test the standardized aseptic technology of permeable cryoprotectant-free vitrification of human spermatozoa in capillaries (for intracytoplasmic sperm injection [ICSI] or in vitro fertilization [IVF]). To test the effect of vitrification on basic sperm parameters, each of 68 swim-up-prepared ejaculates from oligo-astheno-terato-zoospermic patien...

background: spermatozoa cryopreservation is used for the management of infertility and some other medical conditions. the routinely applied cryopreservation technique depends on permeating cryoprotectants, whose toxic effects have raised the attention towards permeating cryoprotectants-free vitrification technique. objective: to compare between the application of slow cryopreservation and vitri...

BACKGROUND Spermatozoa cryopreservation is used for the management of infertility and some other medical conditions. The routinely applied cryopreservation technique depends on permeating cryoprotectants, whose toxic effects have raised the attention towards permeating cryoprotectants-free vitrification technique. OBJECTIVE To compare between the application of slow cryopreservation and vitri...

Abstract The African lion is an excellent model species for the highly endangered Asiatic lion. lions reproduce well in zoos, leading to fact that occasionally ovaries and testis are available in-vitro experiments. We previously performed maturation (IVM) fertilization of oocytes were able produce advanced embryos after intracytoplasmic sperm injection (ICSI) with cryopreserved sperm. Here we e...

Since that historically the first human birth was registered through in vitro fertilization in 1978, the improvement and creation of new assisted reproduction techniques such as intracytoplasmic sperm injection and physiological intracytoplasmic sperm injection have become of great importance nowadays. Louise Brown was the first baby conceived through in vitro fertilization in England. Since th...

Twentieth-century researcher Ernest John Christopher Polge studied the reproductive processes of livestock and determined a method to successfully freeze, thaw, and utilize viable [4] sperm [5] cells to produce offspring in animals. In 1949, Polge identified glycerol as a cryoprotectant, or a medium that enables cells to freeze without damaging their cellular components or functions. Several ye...