OBJECTIVE The goal of this study was to compare the semen parameters of two successive samples obtained within an interval of less than 60 minutes from patients planning to undergo intrauterine insemination (IUI) whose first samples exhibited low semen quality. METHODS Thirty-two consecutive patients were enrolled in the study. On the day of IUI, the semen analysis of the samples initially pr...

The Franklin and Dukes (FD) test is used as a screening test for the determination of sperm-agglutinating antibodies and as a proof of therapeutic effects in cases of infertility, but various parameters of the FD test remain unclear and the application of different techniques has revealed highly different results. To test the statistical evidence for the FD technique, conditions regarded as opt...

The sperm mobility assay used in the present study measures the rate of sperm penetration in a biologically inert cell-separation solution (Accudenz). When a sample of sperm is overlaid in a cuvette containing Accudenz, sperm penetrate the solution and absorbance of the sample can be measured with a spectrophotometer. This assay has been successfully used to select chicken and turkey semen dono...

Background Sperm preparation before assisted reproductive techniques is very important for obtaining successful fertilization and pregnancy. During ICSI technique, sperms are selected based on viability and morphology. But this selection did not guarantee integrity of sperm DNA. Therefore, novel sperm selection procedures are introduced for selection of sperm with low DNA damage. One of these p...

The yield of flow cytometric sorted Xand Y-chromosome-bearing sperm in a given time period is an important factor in the strategies used for fertilization and the production of sex-preselected offspring. This yield is dependent on the efficiency with which the modified flow cytometer/cell sorter analyzes the DNA of spermatozoa. The efficiency is directly related to the number of sperm with the ...

Sperm viability (SV), the proportion of live sperm in a sample, is a widely applied measure of sperm quality but few studies test its robustness. At least three reasons make SV problematic as a surrogate for sperm quality. First, reviewing the ecological literature revealed that previously identified methodological pitfalls have not been overcome, including low cross-study standardization of pr...

Sperm proteomics is the identification and functional study of sperm proteins. It is based on the separation of proteins to generate a sample suitable for mass spectrometry and subsequent protein identification. Various proteomic approaches can be employed to study sperm proteins. Currently it has led to the identification and cataloging of thousands of sperm proteins. Ultimately, the goal is t...

Artificial insemination is a well-established part of modern agricultural practice. A viable semen sample is judged by the total number of spermatozoa (sperm) in the sample and the motility of the sperm. In this paper, we report the development of a reusable measurement cell and electronics for screening semen samples based on the Quartz Crystal Microbalance (QCM) and Universal Frequency to Dig...

The objective of the research was to test the effect of iron and aluminium coagulants commonly used for lake re-cultivation, on the pike sperm motility and fertilisation. The coagulants caused changes in the analyzed hydrochemical parameters of the water such as: Fe, Mg, TSS, SO4 2and Cl. Selected parameters of sperm motility: MOT, VCL, VSL, VAP, LIN were estimated using CASA. The sperm motilit...