BACKGROUND The use of testicular sperm in assisted reproduction depends on the availability of sperm in wet preparations. It is not always possible to recover sperm from the testis, even with previous sperm-positive histopathological findings. The purpose of this study was to evaluate the sperm-negative wet preparation search results with flow cytometric ploidy analysis and histopathological ex...

The selection of sperm cells possessing normal morphology and motility is crucial for many assisted reproductive technologies (ART), especially for intracytoplasmic sperm injection (ICSI), as sperm quality directly affects the probability of inducing healthy pregnancy. We present a novel platform for real-time quantitative analysis and selection of individual sperm cells without staining. Towar...

background: sperm dna fragmentation is known as an important cause of male infertility. the influence of sperm dna damage on reproductive potential has been subject of many studies indicating various results and remaining the subject controversial. in this study, we investigated differences of the semen parameters and intracytoplasmic sperm injection (icsi) outcome according to sperm dna fragme...

CONTEXT Air pollution is common in all countries and affects reproductive functions in men and women. It particularly impacts sperm parameters in men. This meta-analysis aimed to examine the impact of air pollution on the quality of sperm. EVIDENCE ACQUISITION The scientific databases of Medline, PubMed, Scopus, Google scholar, Cochrane Library, and Elsevier were searched to identify relevant...

OBJECTIVE To determine the relationship between sperm DNA methylation level and sperm characteristics and pregnancy rates. DESIGN Prospective study. Quantitation by image analysis of DNA methylation in sperm nucleus. SETTING Department of Reproduction Biology, Edouard Herriot Hospital, Lyon, France. PATIENT(S) Infertile couples undergoing IVF-ET. INTERVENTION(S) The immunostaining of 5 ...

OBJECTIVE To investigate DNA fragmentation by using terminal deoxyribonucleotidyl transferase-mediated dUTP nick-end labeling in relation to World Health Organization parameters and computer-aided sperm analysis (CASA) in sperm to determine the possibility of obtaining a correlation among CASA parameters, sperm morphology, and DNA fragmentation. DESIGN Sperm analysis according to World Health...

The adaptive significance of variation in sperm phenotype is still largely unknown, in part due to the difficulties of observing and measuring sperm movement in its natural, selective environment (i.e., within the female reproductive tract). Computer-assisted sperm analysis systems allow objective and accurate measurement of sperm velocity, but rely on being able to track individual sperm, and ...

Preoperative and postoperative sperm parameter values from infertile men with varicocele were analyzed by computer-aided sperm analysis (CASA) to assess if sperm characteristics improved after varicocelectomy. Semen samples of men with proven fertility (n = 38) and men with varicocele-related infertility (n = 61) were also analyzed. Conventional semen analysis was performed according to WHO (20...

As mobile phone usage is growing rapidly, there is a need for a comprehensive analysis of the literature to inform scientific debates about the adverse effects of mobile phone radiation on sperm quality traits. Therefore, we conducted a meta-analysis of the eligible published research studies on human males of reproductive age. Eleven studies were eligible for this analysis. Based on the meta-a...

A 2-step clustering procedure, using indexes derived from principal component analysis, was used to disclose sperm subpopulations within the canine ejaculate and its relationship to sperm cryoresistance. Semen from 4 dogs was frozen-thawed by a standard protocol: before freezing, computer-assisted sperm analysis of motility and morphometry were performed; after thawing, motility analysis was pe...