The spectrophotometric and microbiological assay methods used for the determination of ampicillin levels in ampicillin/cloxacillin combinations were compared. A total of 24 samples of ampicillin/cloxacillin suspensions from 8 batches and 3 companies (B2, D and E) were assayed for their ampicillin content levels using the Spectrophotometric assay method. Infra-red (IR) Spectroscopy confirmed the...

Micrococcus luteus was employed for the assay of rifampicin in fixed-dose combinations (FDCs) of anti-tubercular drugs containing rifampicin, isoniazid, pyrazinamide and ethambutal. The microbiological assay was compared with spectrophotometric assay for estimation of rifampicin in the FDCs as well as in dissolution studies of the FDCs. The microbiological method was found to be more sensitive ...

To develop a new more accurate spectrophotometric method for detecting monoamine oxidase inhibitors from plant extracts, a series of amine substrates were selected and their ability to be oxidized by monoamine oxidase was evaluated by the HPLC method and a new substrate was used to develop a peroxidase-linked spectrophotometric assay. 4-(Trifluoromethyl) benzylamine (11) was proved to be an exc...

To develop a new more accurate spectrophotometric method for detecting monoamine oxidase inhibitors from plant extracts, a series of amine substrates were selected and their ability to be oxidized by monoamine oxidase was evaluated by the HPLC method and a new substrate was used to develop a peroxidase-linked spectrophotometric assay. 4-(Trifluoromethyl) benzylamine (11) was proved to be an exc...

The Limulus lysate assay was used to measure the endotoxin content in stream water and was found to reflect the degree of bacterial contamination as measured by coliform, enteric, gram-negative, and heterotrophic bacteria. The firm-clot method was found to be a less sensitive and reproducible technique for the detection of endotoxin than was the spectrophotometric modification of the Limulus ly...

OBJECTIVES To assay theophylline blood levels in a sample of Sri Lankan chronic asthmatics taking oral theophylline, and to evaluate a simple and cost effective ultraviolet spectrophotometric assay for theophylline levels in blood. SETTING Chronic asthmatics taking oral theophylline attending medical clinics at the National Hospital of Sri Lanka (NHSL) were recruited for the study. Blood samp...