Because of the primary aminic character of asulam, it is readily labelled with fluorescamine. A study of the variables affecting the derivatization reaction has been made. Two analytical approaches, that is, direct, synchronous spectrofluorimetry have been tested, and they both show good analytical performances. The linear dynamic range of the synchronous approach is 0.043-0.214 mug/ml and the ...

objective(s):cefixime (cfx), is a semi-synthetic third-generation oral cephalosporin antibiotic that is prescribed for the treatment of susceptible infections. there are some procedures for the determination of cfx in pharmaceutical formulations and biological samples. herein a spectrofluorimetric method was proposed for cfx determination based on the fluorescence quenching of terbium-danofloxa...

Ethidium bromide (EtBr) is the most widely used fluorescent dye in nucleic acid gel electrophoresis since decades. However it has been essentially forgotten in DNA quantification by spectrofluorimetry. While investigating sensitivity and dynamic range of available fluorochromes, we found that EtBr permits much more sensitive fluorimetric measurements than previously thought. We report here a re...

This paper deals with a very common problem on spectrofluorimetry: the inner-filter effect. This limitation has made spectrofluorimetry into a disadvantageous technique for the quantification of individual compounds in complex mixtures, however, a lot of papers have quantified compounds as fluorene in mixtures where other Polycyclic Aromatic Hydrocarbons (PAHs) absorb part of the energy emitted...

Two rapid, simple, sensitive, selective and economic derivative spectrophotometric (first [D1] and second [D2]) and synchronous spectrofluorimetric (FDSFS and SDSFS) methods have been developed for the analysis of fexofenadine hydrochloride (FXD) in the presence of its different degradation products. Derivative spectrophotometry (D1) was used to measure FXD at 223 nm in the presence of its alka...

Three new, simple, sensitive and selective spectrofluorimetric and spectrophotometric methods were developed for the determination of the γ-amino-n-butyric acid derivative, pregabalin. Pregabalin as a primary amine reacts with fluorescamine to yield a fluorescent product (Method I), with 2,4-dinitrofluorobenzene (Method II) and 2,3,5,6-tetrachloro-1,4-benzoquinone (Method III) in aqueous alkali...