the method of quantitative competitive polymerase chain reaction (qc-pcr) was conducted to compare the number of rumen anaerobic fungi in pure culture of fungi containing high fat sunflower meal (165 g fat/kg dm) processed with formaldehyde and naoh. twenty one multiparous early lactating holstein cows (30±5 days of lactation) selected and fed experimental diets for 7 weaks. the diets were incl...

Proteolytic, aminopeptidase, endopeptidase and carboxypeptidase activities of seven strains of rumen anaerobic fungi, selected to represent the fungal population commonly found in the rumen, were investigated in vitro. Whatever the nitrogen source included in the culture medium, a proteolytic activity against the 14C-labelled casein was detected in only one fungal strain. This strain belonged t...

background and objectives: essential oils may improve the utilization of nutrients by ruminal microorganisms. the aim of this study was to assess the effect of different doses of ajowan essential oils (aeo) on growth and fibrolytic activity of anaerobic fungi, and generic distribution of ciliated protozoa ( in vitro ). material and methods: different doses of aeo (0, 150, 300, 450 and 600 ppm) ...

Anaerobic fungi are key players in the breakdown of fibrous plant material in the rumen, but not much is known about the composition and stability of fungal communities in ruminants. We analyzed anaerobic fungi in 53 rumen samples from farmed sheep (4 different flocks), cattle, and deer feeding on a variety of diets. Denaturing gradient gel electrophoresis fingerprinting of the internal transcr...

Three polycentric rumen fungi, LL, LC2 and Ruminomyces elegans (C2), isolated from the rumen of cattle were grown in six culture media. LL and LC2 were morphologically similar. Their characteristics resembled those of Orpinomyces and Neocallimastix joyonii, and they grew well and produced sporangia after 3-4 d growth in all the media. R. elegans differed morphologically from LL and LC2, but alt...

The status of anaerobic zoosporic (Chytridiomycota) fungi along the entire digestive tract of sheep was assessed both analytically and microscopically. Digest samples were taken from different segments of the digestive tracts of three newly killed sheep that previously had been used in experimental dietary studies. These digest samples were tested for the presence of rumen fungi by assessing th...

Large populations of obligately anaerobic fungi colonise plant fragments in the rumen of cattle and sheep on fibrous diets. Highest numbers are present on stem fragments and the fungi are found associated mainly with the lignified tissues that remain in the rumen for prolonged periods. Fungal populations are highest in animals receiving high-fibre diets and are absent from the rumen contents of...

the aim of this study was to evaluate the effect of substitution of soybean meal with canola meal and measure its effects on rumen anaerobic fungal population in lactation iranian of holstein cows. from d 5 to 56 postpartum, cows were fed diets that were isoenergetic containing soybean meal (sbm; n = 3) or canola meal (cm; n = 3). cows were housed in tie stalls and fed tmr two times a day. comp...

The recent recognition of fungi in the oceanic igneous crust challenges the understanding of this environment as being exclusively prokaryotic and forces reconsiderations of the ecology of the deep biosphere. Anoxic provinces in the igneous crust are abundant and increase with age and depth of the crust. The presence of anaerobic fungi in deep-sea sediments and on the seafloor introduces a type...