نتایج جستجو برای: southern blot hybridization
تعداد نتایج: 211655 فیلتر نتایج به سال:
A. pleuropneumoniae. We tested this by electrotransformation of A. pleuropneumoniae 4074 with pHN61 as described above. In this transformant, the MICs of lincomycin and clindamycin increased from 16 to 128 mg/L and 4 to 64 mg/L respectively, whereas the MIC of erythromycin was unchanged (1 mg/L). The presence of pHN61 was verified by plasmid extraction and Southern blot hybridization. These res...
References: 1) Oshima,A. et al. (1988) J. Biol. Chem. 263, 2553-2562. 2) Laureys.G. etal. (1988) Genomicsi, 224-229. 2/162 6/162 129/162 20/162 1/162 4/162 0.01 0.04 0.80 0.12 0.01 0.02 Source/Description: The clone VAW140 (1) was tested for CA repeats by Southern blot hybridization (2). One CA-hybridizing Hindm fragment was subcloned into mp8 in both orientations and sequenced at each end. A (...
Our method extends the Southern blotting technique to a new degree of sensitivity . \.J'e use a radioactive probe not just to identify a single r estriction fragment of DNA but as a way of end-labeling a unique restriction fragment. This endlabel ing by hybridization is analogous to the endlabeling that we do in the conventional DNA sequencing , which enables us to display in any partial di ges...
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The presence of recombinant DNA in soil cultivated with white poplars (Populus alba L.) expressing either the bar transgene for herbicide tolerance or the StSy transgene for resveratrol production, respectively, was investigated in a greenhouse over a 20-month period. The bar trial included the transgenic lines 5P56 and 6EA22P56 and the untransformed line, while the StSy trial was established w...
Restriction endonuclease analysis of the ilvGEDA operon of members of the family Enterobacteriaceae.
Four of the genes required for the biosynthesis of isoleucine and valine form the ilvGEDA operon in Escherichia coli K-12 and Salmonella typhimurium. The structural relationship of these genes was examined in eight other members of the family Enterobacteriaceae by genomic Southern blot hybridization. These genes are contiguous in all the strains examined, and specific restriction sites appear t...
IncP-1, IncP-7 and IncP-9 plasmids often carry genes encoding enzymes involved in the degradation of man-made and natural contaminants, thus contributing to bacterial survival in polluted environments. However, the lack of suitable molecular tools often limits the detection of these plasmids in the environment. In this study, PCR followed by Southern blot hybridization detected the presence of ...
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